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Browsing by Author "Gallagher, Joe A."

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Absence of turnover and futile cycling of sucrose in leaves of Lolium temulentum L.: implications for metabolic compartmentation

Cairns, Andrew J.; Gallagher, Joe A. (2004-05-12)

To study the interdependence of sucrose accumulation and its hydrolyzing enzyme, soluble acid invertase (AI; EC 3.2.1.26), in fructan-accumulating temperate grasses and cereals, experiments were performed in which sucrose ...
Approaches for associating molecular polymorphisms with phenotypic traits based on linkage disequilibrium in natural populations of Lolium perenne

Humphreys, Janet; Skøt, Leif; Humphreys, Mervyn O.; Thomas, Ian D.; Gallagher, Joe A.; Armstead, Ian P. (Wageningen Academic Publishers, 2001)

Grassland covers 26% of the world's total land area. It produces feed for livestock; maintains soil fertility; protects and conserves soil and water resources; creates a habitat for wildlife; provides recreational space ...
Balancing supply and demand: the spatial regulation of carbon metabolism in grass and cereal leaves

Gallagher, Joe A.; Pollock, Christopher J.; Tomos, A. Dewi; Farrar, John F.; Koroleva, Olga A.; Lu, C. (2003-01-01)

Leaf primary metabolism responds to changes in both supply of inputs and demand for products. Metabolic control in leaves changes both spatially and temporally. Using leaves of C3 temperate Gramineae, the spatial control ...
Carbon allocation and sugar status in individual cells of barley leaves affects expression of sucrose:fructan 6-fructosyltransferase gene

Farrar, John F.; Koroleva, Olga A.; Pollock, Christopher J.; Tomos, A. Deri; Gallagher, Joe A. (2001)

Heterogeneity of function in plant tissues requires precise regulation of metabolic systems controlling resource allocation at the level of single cells. Differential distribution of metabolites between epidermis, mesophyll ...
Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L.

Cairns, Andrew J.; Pollock, Christopher J.; Gallagher, Joe A. (2004-03-01)

The invertases of Lolium temulentum have been characterized at the enzyme level. However, studies on the expression of the genes coding for these enzymes have been lacking. To elucidate the role of acid ‘invertase-like’ ...
Functional genomics of forage and bioenergy quality traits in the grasses

Donnison, Iain S.; Farrar, Kerrie; Allison, Gordon G.; Hodgson, Edward; Adams, Jessica M.; Hatch, Robert; Gallagher, Joe A.; Robson, Paul R.; Clifton-Brown, John C.; Morris, Phillip (2009)

Biomass from forage and energy crops can provide a renewable source of meat, milk, and wool, or power, heat, transport fuels and platform chemicals, respectively. Whilst in forage grasses some improvements have been made, ...
Genetic variation in natural populations of perennial ryegrass in relation to flowering time and soluble carbohydrates

Sanderson, Ruth; Thomas, Ian D.; Skøt, Kirsten P.; Skøt, Leif; Gallagher, Joe A.; Heywood, Susan; Armstead, Ian P.; Humphreys, Mervyn O. (2004)
Kinetic constants of signal peptidase I using cytochrome b5 as a precursor substrate

Kaderbhai, Naheed N.; Kaderbhai, Mustak A.; Gallagher, Joe A. (2001-11-26)

A procedure is described for measuring Escherichia coli signal peptidase I activity which exploits an intact precursor protein composed of the alkaline phosphatase signal peptide fused to the full length mammalian cytochrome ...
Mapping water-soluble carbohydrate content in perennial ryegrass

Gallagher, Joe A.; Humphreys, Mervyn O.; Cairns, Andrew J.; Turner, Lesley B.; Armstead, Ian P. (Wageningen Academic Publishers, 2005)

Grassland covers 26% of the world's total land area. It produces feed for livestock; maintains soil fertility; protects and conserves soil and water resources; creates a habitat for wildlife; provides recreational space ...
Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light

Farrar, John F.; Pollock, Christopher J.; Lu, Chungui; Koroleva, Olga A.; Tomos, A. Deri; Gallagher, Joe A. (2002-11-01)

We describe a highly efficient two-step single-cell reverse transcriptase-polymerase chain reaction technique for analyzing gene expression at the single-cell level. Good reproducibility and a linear dose response indicated ...
Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Pollock, Christopher J.; Koroleva, Olga A.; Farrar, John F.; Tomos, A. D.; Gallagher, Joe A. (2001)

A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) ...

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