Browsing by Author "Kelly, Diane E."

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  • Venkateswarlu, K.; Lamb, David C.; Warrilow, Andrew G. S.; Kelly, Diane E.; Kelly, Steven L. (2001-08-10)
    Native yeast NADPH–cytochrome P450 oxidoreductase (CPR; EC 1.6.2.4) and a soluble derivative lacking 33 amino acids of the NH2-terminus have been overexpressed as recombinant proteins in Escherichia coli. The presence of ...
  • Kelly, Steven L.; Lamb, David C.; Jackson, Colin J.; Warrilow, Andrew G. S.; Kelly, Diane E. (Academic Press, 2003)
    The cytochrome P450 (CYP) superfamily of genes and proteins are well known for their involvement in pharmacology and toxicology, but also increasingly for their importance and diversity in microbes. The extent of diversity ...
  • Jackson, Colin J.; Parker, Josie E.; Lamb, David C.; Marczylo, Timothy H.; Kelly, Diane E.; Manning, Nigel L.; Kelly, Steven L. (2003-02-07)
    The genetic locus encoding cytochrome P450 51 (CYP51; P45014DM) in Mycobacterium smegmatis is described here together with confirmation of activity in lanosterol 14α-demethylation. The protein bound azole antifungals with ...
  • Kelly, Steven L.; Waterman, M. R.; Skaug, T.; Jackson, Colin J.; Kelly, Diane E.; Podust, L. M.; Kell, Douglas B.; Song, H. L.; Lamb, David C. (2002-04-09)
    In the present study we describe the complete cytochrome P450 complement, the “CYPome,” of Streptomyces coelicolor A3(2). Eighteen cytochromes P450 (CYP) are described, in contrast to the absence of CYPs in Escherichia ...
  • Giaever, Guri; Chu, Angela M.; Ni, Li; Connelly, Carla; Riles, Linda; Véronneau, Steeve; Dow, Sally; Lucau-Danila, Ankuta; Anderson, Keith; André, Bruno; Arkin, Adam P; Astromoff, Anna; El Bakkoury, Mohamed; Bangham, Rhonda; Benito, Rocio; Brachatt, Sophie; Campanaro, Stefano; Curtiss, Matt; Davis, Karen; Deurschbauer, Adam; Entian, Karl-Dieter; Flaherty, Patrick; Foury, Francoise; Garfinkel, David; Gerstein, Mark; Gotte, Deanna; Güldener, Ulrich; Hegemann, Johannes H.; Hempel, Svenga; Herman, Zelek; Jaramillo, Daniel F.; Kelly, Diane E.; Kelly, Steven L.; Kötter, Steven; LaBont, Darlene; Lamb, David C.; Lan, Ning; Liang, Hong; Liao, Hong; Liu, Lucy; Luo, Chuanyun; Lussier, Marc; Mao, Rong; Menard, Patrice; Ooi, Siew Loon; Revuelta, Jose L.; Roberts, Christopher J.; Rose, Matthias; Ross-Macdonald, Petra; Scherens, Bart; Schimmack, Greg; Schafer, Brenda; Shoemaker, Daniel D.; Sookhai-Mahadeo, Sharon; Storms, Reginald K.; Strathern, Jeffrey N.; Valle, Giorgio; Voet, Marleen; Volckaert, Guido; Wang, Chin-yun; Ward, Teresa R.; Wilhelmy, Julie; Winzeler, Elizabeth A.; Yang, Yonghong; Yen, Grace; Youngman, Elaine; Yu, Kexin; Bussey, Howard; Boeke, Jef D.; Snyder, Michael; Philippsen, Peter; Davis, Ronald W.; Johnston, Mark (2002-07-25)
    Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically ...
  • Lamb, David C.; Kelly, Steven L.; Kelly, Diane E. (2001)
    In the year 2001 a collection of yeast strains will be completed that are deleted in the 6000 open reading frames selected as putative genes by the initial bioinformatic analysis of the Saccharomyces cerevisiae genome. The ...
  • Venkateswarlu, K.; Kelly, Steven L.; Kaderbhai, Naheed N.; Lamb, David C.; Kaderbhai, Mustak A.; Kelly, Diane E. (2001-11-01)
    Human sterol 14α-demethylase (P45051; CYP51) catalyzes the oxidative removal of the C32 methyl group of dihydrolanosterol, an essential step in the cholesterol biosynthetic pathway. The reaction is dependent upon NADPH ...
  • Groeneveld, Philip; Rolley, Nicola; Kell, Douglas B.; Kelly, Diane E. (2002)
    Sterols are essential components of eukaryotic membranes and contribute to important physiological characteristics, such as osmotic robustness and hormonal effects on growth (Daum et al., 1999), but most importantly, the ...
  • Clark, A.; Kelly, Diane E. (2003)
    The raison d'ítre for the drug discovery and development process is to provide safe and effective treatments for diseases. Bringing a new drug to market, however, is a time-consuming and expensive process and it remains ...
  • Lamb, David C.; Manning, Nigel J.; Jackson, Colin J.; Kelly, Diane E.; Kelly, Steven L. (2003-10-03)
    Understanding fluconazole resistance is important as it emerged as a serious clinical problem for this CYP51, sterol 14α-demethylase, inhibitor. One mechanism, observed first in Saccharomyces cerevisiae, was through defective ...
  • Marczylo, Timothy H.; Kelly, Diane E.; Kelly, Steven L.; Lamb, David C.; Lowe, David J.; Manning, Nigel J.; Warrilow, Andrew G. S.; Jackson, Colin J. (2002-09-06)
    Sterol 14α-demethylase encoded by CYP51 is a member of the cytochrome P450 (CYP) superfamily of enzymes and has been shown to have an essential role in sterol biosynthesis in eukaryotes, with orthologues recently being ...
  • Kelly, Steven L.; Lamb, David C.; Marczylo, Timothy H.; Jackson, Colin J.; Kelly, Diane E.; Cannieux, Michel; Ugochukwu, C.; Greetham, Darren (2001)
    Cytochrome P450 51 (CYP51) is sterol 14a-demethylase, known also as Erg11p in yeast. First studied in yeast, where it is one of three CYPs in the genome, it has subsequently gained attention as the only CYP found so far ...
  • Kelly, Steven L.; Lamb, David C.; Warrilow, Andrew G. S.; Kelly, Diane E. (2002-11-29)
    The recently completed genome of the basidiomycete, Phanerochaete chrysosporium, revealed the presence of one NADPH-cytochrome P450 oxidoreductase (CPR; EC 1.6.2.4) gene and >123 cytochrome P450 (CYP) genes. How a single ...
  • Lamb, David C.; Kelly, Diane E.; Bak, Soren; Warrilow, Andrew G. S.; Kelly, Steven L.; Cannieux, Michel; Kahn, Rachel A.; Manning, Nigel J. (2001-06-15)
    Azole fungicides were thought to have much greater affinity for the fungal cytochrome P450 enzyme, sterol 14α-demthylase (CYP51) than the plant orthologue. Using purified CYP51 from the plant Sorghum bicolor L Moenech, a ...
  • Kelly, Steven L.; Kelly, Diane E. (2003-02)
    You can’t teach an old dog new tricks (so the adage goes). But it appears that baker’s yeast is more accommodating. In one of the most comprehensive metabolic engineering feats ever accomplished, a report in this issue1 ...
  • Fowler, K.; Manning, Nigel J.; Lamb, David C.; Kelly, Diane E.; Kieser, T.; Podust, L. M.; Waterman, M. R.; Kelly, Steven L. (2002)
    The annotation of the genome sequence of Streptomyces coelicolor A3(2) revealed a cytochrome P450 (CYP) resembling various sterol 14a-demethylases (CYP51). The putative CYP open reading frame (SC7E4.20) was cloned with a ...