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Browsing by Author "Lee, David"

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Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences

Lee, David; La Mura, Maurizio; Allnutt, Theo R.; Powell, W. (2009-02-02)

The most common method of GMO detection is based upon the amplification of GMO-specific DNA amplicons using the polymerase chain reaction (PCR). Here we have applied the loop-mediated isothermal amplification (LAMP) method ...
G-string slippage turns white rice red

Powell, Wayne; Lupotto, Elisabetta; Lee, David (2009-05-02)

The mutations that convey the white pericarp phenotype to rice (Oryza sativa subsp. japonica) are in a regulatory gene, Rc. We have identified a genetic difference between the cultivar ‘Perla’ and its natural red rice ...
Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping

Lee, David; La Mura, Maurizio; Allnutt, Theo; Powell, W.; Greenland, Andy (2009-09-22)

DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification ...
Molecular Markers- History, Features and Application

Doveri, S.; Powell, Wayne; Maheswaran, M.; Lee, David (Science Publishers, 2007)

Genome of an organism is depicted by genetic linkage mapping and physical mapping. Genome mapping stated with genetic linkage mapping and contributed enormously in genome analysis and its improvement. Physical mapping ...

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