A demonstration of a 1 : 1 correspondence between chiasma frequency and recombination using a Lolium perenne/Festuca pratensis substitution

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dc.contributor.author King, Julie
dc.contributor.author Roberts, Luned A.
dc.contributor.author Kearsey, Mike J.
dc.contributor.author Thomas, Huw M.
dc.contributor.author King, Ian P.
dc.contributor.author Jones, R. Neil
dc.contributor.author Huang, Lin Shiow-Fen
dc.contributor.author Armstead, Ian Peter
dc.contributor.author Morgan, W. Gareth
dc.contributor.author King, Ian Philip
dc.date.accessioned 2008-12-04T15:07:22Z
dc.date.available 2008-12-04T15:07:22Z
dc.date.issued 2002-05-01
dc.identifier.citation King , J , Roberts , L A , Kearsey , M J , Thomas , H M , King , I P , Jones , R N , Huang , L S-F , Armstead , I P , Morgan , W G & King , I P 2002 , ' A demonstration of a 1 : 1 correspondence between chiasma frequency and recombination using a Lolium perenne/Festuca pratensis substitution ' Genetics , vol 161 , no. 1 , pp. 307-314 . en
dc.identifier.issn 0016-6731
dc.identifier.other PURE: 94039
dc.identifier.other dspace: 2160/1353
dc.identifier.uri http://hdl.handle.net/2160/1353
dc.identifier.uri http://www.genetics.org/cgi/content/abstract/161/1/307 en
dc.description King, J., Roberts, L. A., Kearsey, M. J., Thomas, H. M., Jones, R. N., Huang, L., Armstead, I. P., Morgan, W. P., King, I. P. (2002). A demonstration of a 1:1 correspondence between chiasma frequency and recombination using a Lolium perenne/Festuca pratensis substitution. Genetics, 161 (1), 307-314 Sponsorship: BBSRC RAE2008 en
dc.description.abstract A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line . The chromatin of F. pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Genetics en
dc.title A demonstration of a 1 : 1 correspondence between chiasma frequency and recombination using a Lolium perenne/Festuca pratensis substitution en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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