Show simple item record Gustavo en_US Russell M. en_US Joseph V. en_US Alessandra L. en_US E. James en_US John en_US David A. en_US Peter Michael en_US 2008-12-04T17:04:32Z 2008-12-04T17:04:32Z 2006-12 en_US
dc.identifier en_US
dc.identifier.citation Chemale , G , Morphew , R M , Moxon , J V , Morassuti , A L , LaCourse , E J , Barrett , J , Johnston , D A & Brophy , P M 2006 , ' Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica ' Proteomics , vol 6 , no. 23 , pp. 6263-6273 . , 10.1002/pmic.200600499 en_US
dc.identifier.other PURE: 93980 en_US
dc.identifier.other dspace: 2160/1387 en_US
dc.description.abstract The parasite Fasciola hepatica causes major global disease of livestock, with increasing reports of human infection. Vaccine candidates with varying protection rates have been identified by pre-genomic approaches. As many candidates are part of protein superfamilies, sub-proteomics offers new possibilities to systematically reveal the relative importance of individual family proteins to vaccine formulations within populations. The superfamily glutathione transferase (GST) from liver fluke has phase II detoxification and housekeeping roles, and has been shown to contain protective vaccine candidates. GST were purified from cytosolic fractions of adult flukes using glutathione- and S-hexylglutathione-agarose, separated by 2-DE, and identified by MS/MS, with the support of a liver fluke EST database. All previously described F. hepatica GST isoforms were identified in 2-DE. Amongst the isoforms mapped by 2-DE, a new GST, closely related to the Sigma class enzymes is described for the first time in the liver fluke. We also describe cDNA encoding putative Omega class GST in F. hepatica. en_US
dc.format.extent 11 en_US
dc.relation.ispartof Proteomics en_US
dc.subject 2-DE en_US
dc.subject F. hepatica en_US
dc.subject Glutathione transferases en_US
dc.subject Omega en_US
dc.subject Sigma en_US
dc.title Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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