Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica

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dc.contributor.author Chemale, Gustavo
dc.contributor.author Morphew, Russell M.
dc.contributor.author Moxon, Joseph V.
dc.contributor.author Morassuti, Alessandra L.
dc.contributor.author LaCourse, E. James
dc.contributor.author Barrett, John
dc.contributor.author Johnston, David A.
dc.contributor.author Brophy, Peter Michael
dc.date.accessioned 2008-12-04T17:04:32Z
dc.date.available 2008-12-04T17:04:32Z
dc.date.issued 2006-12
dc.identifier.citation Chemale , G , Morphew , R M , Moxon , J V , Morassuti , A L , LaCourse , E J , Barrett , J , Johnston , D A & Brophy , P M 2006 , ' Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica ' Proteomics , vol 6 , no. 23 , pp. 6263-6273 . en
dc.identifier.issn 1615-9853
dc.identifier.other PURE: 93980
dc.identifier.other dspace: 2160/1387
dc.identifier.uri http://hdl.handle.net/2160/1387
dc.identifier.uri http://www3.interscience.wiley.com/cgi-bin/abstract/113447914/ en
dc.description Chemale, G., Morphew, R. M., Moxon, J. V., Morassuti, A. L., LaCourse, E. J., Barrett, J., Johnston, D. A., Brophy, P. M. (2006). Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica. Proteomics, 6 (23), 6263-6273 Sponsorship: BBSRC (BB/C503638/1)/ EU (STREP DELIVER);BBSRC UK; Grant Number: BB/C503638/1: EU(STREP DELIVER) RAE2008 en
dc.description.abstract The parasite Fasciola hepatica causes major global disease of livestock, with increasing reports of human infection. Vaccine candidates with varying protection rates have been identified by pre-genomic approaches. As many candidates are part of protein superfamilies, sub-proteomics offers new possibilities to systematically reveal the relative importance of individual family proteins to vaccine formulations within populations. The superfamily glutathione transferase (GST) from liver fluke has phase II detoxification and housekeeping roles, and has been shown to contain protective vaccine candidates. GST were purified from cytosolic fractions of adult flukes using glutathione- and S-hexylglutathione-agarose, separated by 2-DE, and identified by MS/MS, with the support of a liver fluke EST database. All previously described F. hepatica GST isoforms were identified in 2-DE. Amongst the isoforms mapped by 2-DE, a new GST, closely related to the Sigma class enzymes is described for the first time in the liver fluke. We also describe cDNA encoding putative Omega class GST in F. hepatica. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Proteomics en
dc.subject 2-DE en
dc.subject F. hepatica en
dc.subject Glutathione transferases en
dc.subject Omega en
dc.subject Sigma en
dc.title Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1002/pmic.200600499
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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