Laser desorption/ionization mass spectrometry on porous silicon for metabolome analyses: influence of surface oxidation

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dc.contributor.author Jones, D. G.
dc.contributor.author Vaidyanathan, S.
dc.contributor.author Jenkins, Tudor
dc.contributor.author Ellis, J.
dc.date.accessioned 2008-12-09T09:40:08Z
dc.date.available 2008-12-09T09:40:08Z
dc.date.issued 2007-07-15
dc.identifier.citation Jones , D G , Vaidyanathan , S , Jenkins , T & Ellis , J 2007 , ' Laser desorption/ionization mass spectrometry on porous silicon for metabolome analyses: influence of surface oxidation ' Rapid Communications in Mass Spectrometry , vol 21 , no. 13 , pp. 2157-2166 . en
dc.identifier.issn 0951-4198
dc.identifier.other PURE: 89352
dc.identifier.other dspace: 2160/1464
dc.identifier.uri http://hdl.handle.net/2160/1464
dc.identifier.uri http://www3.interscience.wiley.com/cgi-bin/abstract/114276131/ABSTRACT en
dc.description Jenkins, Tudor; Vaidyanathan, S.; Jones, D.G.; Ellis, J., (2007) 'Laser desorption/ionization mass spectrometry on porous silicon for metabolome analyses: influence of surface oxidation', Rapid Communications in Mass Spectrometry 21(13) pp.2157-2166 RAE2008 en
dc.description.abstract Laser desorption/ionization mass spectrometry (LDI-MS) on porous silicon is a promising analytical strategy for the rapid detection of metabolites in biological matrices. We show that both oxidized and unoxidized porous silicon surfaces are useful in detecting protonated/deprotonated molecules from compounds when analyzed in mixtures. We demonstrate the feasibility of using this technique for the simultaneous detection of multiple analytes using a synthetic cocktail of 30 compounds commonly associated with prokaryotic and eukaryotic primary metabolism. The predominantly detected species were the protonated molecules or their sodium/potassium adducts in the positive-ion mode and the deprotonated molecules in the negative-ion mode, as opposed to fragments or other adducts. Surface oxidation appears to influence mass spectral responses; in particular, in the mixture we studied, the signal intensities of the hydrophobic amino acids were noticeably reduced. We show that whilst quantitative changes in individual analytes can be detected, ion suppression effects interfere when analyte levels are altered significantly. However, the response of most analytes was relatively unaffected by changes in the concentration of one of the analytes, so long as it was not allowed to dominate the mixture, which may limit the dynamic range of this approach. The differences in the response of the analytes when analyzed in mixtures could not be accounted for by considering their gas-phase and aqueous basicities alone. The implications of these findings in using the technique for metabolome analyses are discussed. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Rapid Communications in Mass Spectrometry en
dc.title Laser desorption/ionization mass spectrometry on porous silicon for metabolome analyses: influence of surface oxidation en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1002/rcm.3078
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.contributor.institution Institute of Mathematics & Physics (ADT) en
dc.contributor.institution Mathematics and Physics en
dc.description.status Peer reviewed en


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