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dc.contributor.author Farrar, John F.
dc.contributor.author Pollock, Christopher J.
dc.contributor.author Lu, Chungui
dc.contributor.author Koroleva, Olga A.
dc.contributor.author Tomos, A. Deri
dc.contributor.author Gallagher, Joe A.
dc.date.accessioned 2008-12-09T10:32:32Z
dc.date.available 2008-12-09T10:32:32Z
dc.date.issued 2002-11-01
dc.identifier.citation Farrar , J F , Pollock , C J , Lu , C , Koroleva , O A , Tomos , A D & Gallagher , J A 2002 , ' Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light ' Plant Physiology , pp. 1335-1348 . , 10.1104/pp.008979 en
dc.identifier.issn 1532-2548
dc.identifier.other PURE: 91407
dc.identifier.other dspace: 2160/1474
dc.identifier.uri http://hdl.handle.net/2160/1474
dc.identifier.uri http://www.plantphysiol.org/cgi/reprint/130/3/1335 en
dc.description Chungui Lu, Olga A. Koroleva, John F. Farrar, Joe Gallagher, Chris J. Pollock, and A. Deri Tomos (2002). Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light. Plant Physiology, 130 (3) pp.1335-1348 Sponsorship: BBSRC RAE2008 en
dc.description.abstract We describe a highly efficient two-step single-cell reverse transcriptase-polymerase chain reaction technique for analyzing gene expression at the single-cell level. Good reproducibility and a linear dose response indicated that the technique has high specificity and sensitivity for detection and quantification of rare RNA. Actin could be used as an internal standard. The expression of message for Rubisco small subunit (RbcS), chlorophyll a/b-binding protein (Cab), sucrose (Suc):fructan-6-fructosyl transferase (6-SFT), and Actin were measured in individual photosynthetic cells of the barley (Hordeum vulgare) leaf. Only Actin was found in the non-photosynthetic epidermal cells. Cab, RbcS, and 6-SFT genes were expressed at a low level in mesophyll and parenchymatous bundle sheath (BS) cells when sampled from plants held in dark for 40 h. Expression increased considerably after illumination. The amount of 6-SFT, Cab, and RbcS transcript increased more in mesophyll cells than in the parenchymatous BS cells. The difference may be caused by different chloroplast structure and posttranscriptional control in mesophyll and BS cells. When similar single-cell samples were assayed for Suc, glucose, and fructan, there was high correlation between 6-SFT gene expression and Suc and glucose concentrations. This is consistent with Suc concentration being the trigger for transcription. Together with earlier demonstrations that the mesophyll cells have a higher sugar threshold for fructan polymerization, our data may indicate separate control of transcription and enzyme activity. Values for the sugar concentrations of the individual cell types are reported. en
dc.format.extent 14 en
dc.language.iso eng
dc.relation.ispartof Plant Physiology en
dc.title Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1104/pp.008979
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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