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dc.contributor.author John F. en_US
dc.contributor.author Christopher J. en_US
dc.contributor.author Chungui en_US
dc.contributor.author Olga A. en_US
dc.contributor.author A. Deri en_US
dc.contributor.author Joe A. en_US
dc.date.accessioned 2008-12-09T10:32:32Z
dc.date.available 2008-12-09T10:32:32Z
dc.date.issued 2002-11-01 en_US
dc.identifier http://dx.doi.org/10.1104/pp.008979 en_US
dc.identifier.citation Farrar , J F , Pollock , C J , Lu , C , Koroleva , O A , Tomos , A D & Gallagher , J A 2002 , ' Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light ' Plant Physiology , pp. 1335-1348 . , 10.1104/pp.008979 en_US
dc.identifier.other PURE: 91407 en_US
dc.identifier.other dspace: 2160/1474 en_US
dc.identifier.uri http://hdl.handle.net/2160/1474
dc.description.abstract We describe a highly efficient two-step single-cell reverse transcriptase-polymerase chain reaction technique for analyzing gene expression at the single-cell level. Good reproducibility and a linear dose response indicated that the technique has high specificity and sensitivity for detection and quantification of rare RNA. Actin could be used as an internal standard. The expression of message for Rubisco small subunit (RbcS), chlorophyll a/b-binding protein (Cab), sucrose (Suc):fructan-6-fructosyl transferase (6-SFT), and Actin were measured in individual photosynthetic cells of the barley (Hordeum vulgare) leaf. Only Actin was found in the non-photosynthetic epidermal cells. Cab, RbcS, and 6-SFT genes were expressed at a low level in mesophyll and parenchymatous bundle sheath (BS) cells when sampled from plants held in dark for 40 h. Expression increased considerably after illumination. The amount of 6-SFT, Cab, and RbcS transcript increased more in mesophyll cells than in the parenchymatous BS cells. The difference may be caused by different chloroplast structure and posttranscriptional control in mesophyll and BS cells. When similar single-cell samples were assayed for Suc, glucose, and fructan, there was high correlation between 6-SFT gene expression and Suc and glucose concentrations. This is consistent with Suc concentration being the trigger for transcription. Together with earlier demonstrations that the mesophyll cells have a higher sugar threshold for fructan polymerization, our data may indicate separate control of transcription and enzyme activity. Values for the sugar concentrations of the individual cell types are reported. en_US
dc.format.extent 14 en_US
dc.relation.ispartof Plant Physiology en_US
dc.title Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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