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dc.contributor.author Cairns, Andrew J.
dc.contributor.author Pollock, Christopher J.
dc.contributor.author Gallagher, Joe A.
dc.date.accessioned 2008-12-09T12:08:56Z
dc.date.available 2008-12-09T12:08:56Z
dc.date.issued 2004-03-01
dc.identifier.citation Cairns , A J , Pollock , C J & Gallagher , J A 2004 , ' Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L. ' Journal of Experimental Botany , pp. 557-569 . , 10.1093/jxb/erh056 en
dc.identifier.issn 1460-2431
dc.identifier.other PURE: 91667
dc.identifier.other dspace: 2160/1482
dc.identifier.uri http://hdl.handle.net/2160/1482
dc.identifier.uri http://jxb.oxfordjournals.org/cgi/reprint/55/397/557 en
dc.description J. A. Gallagher, A. J. Cairns and C. J. Pollock (2004). Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L. Journal of Experimental Botany, 55 (397) pp.557-569 Sponsorship: BBSRC RAE2008 en
dc.description.abstract The invertases of Lolium temulentum have been characterized at the enzyme level. However, studies on the expression of the genes coding for these enzymes have been lacking. To elucidate the role of acid ‘invertase-like’ genes in sucrose metabolism and carbon partitioning in Gramineae further, three ‘invertase-like’ homologous clones were isolated from L. temulentum cDNA expression libraries based on leaf tissue, using maize soluble invertase probes. The effect of developmental stage and alterations in carbohydrate status on the expression and tissue distribution of these genes was investigated. The three highly homologous genes (Inv 1:2, Inv 1:4, and FT 2:2) show different patterns of expression and different tissue distribution. Inv 1:2 was predominantly expressed in root tissue. Expression increased during the dark in root and tiller base tissue. Minimal variations in gene expression were observed in leaf tissue following changes in carbohydrate status. Inv 1:4 was predominantly expressed in tiller bases, leaf sheath, and leaf base, with increased expression in tissue samples in the dark period. FT 2:2 was also predominantly expressed in tiller bases, leaf sheath, and leaf base. Higher expression was observed in leaf tissue following increases in carbohydrate content, in a manner that paralleled the regulation and spatial occurrence of fructan in the leaf tissue. Whilst invertases and fructosyltransferases are difficult to distinguish at the level of the whole sequence, analysis of 5' sequence and specific amino acids allows discrimination which correlates with patterns of expression within the tissue. Based on expression patterns and sequence characteristics, it is proposed that Inv 1:2 and Inv 1:4 code for soluble acid invertases, whilst FT 2:2 codes for a fructosyltransferase. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Journal of Experimental Botany en
dc.title Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L. en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1093/jxb/erh056
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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