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dc.contributor.author Murray, Douglas B.
dc.contributor.author Beckmann, Manfred
dc.contributor.author Kitano, Hiroaki
dc.date.accessioned 2008-12-11T12:11:24Z
dc.date.available 2008-12-11T12:11:24Z
dc.date.issued 2007-02-13
dc.identifier.citation Murray , D B , Beckmann , M & Kitano , H 2007 , ' Regulation of yeast oscillatory dynamics ' Proceedings of the National Academy of Sciences of the United States of America , vol 104 , no. 7 , pp. 2241-2246 . , 10.1073/pnas.0606677104 en
dc.identifier.issn 0027-8424
dc.identifier.other PURE: 91802
dc.identifier.other dspace: 2160/1534
dc.identifier.uri http://hdl.handle.net/2160/1534
dc.identifier.uri http://www.pnas.org/cgi/reprint/0606677104v1 en
dc.description Douglas B. Murray, Manfred Beckmann, and Hiroaki Kitano. (2007). Regulation of yeast oscillatory dynamics. Proceedings of the National Academy of Sciences of the USA, 104 (7), 2241-2246 Sponsorship: Solution-Oriented Research for Science and Technology Agency to the Systems Biology Institute /21st Century Center of Excellence Program and Special Coordination Program of the Ministry of Education, Sports, Culture, Science, and Technology to Keio University RAE2008 en
dc.description.abstract When yeast cells are grown continuously at high cell density, a respiratory oscillation percolates throughout the population. Many essential cellular functions have been shown to be separated temporally during each cycle; however, the regulatory mechanisms involved in oscillatory dynamics remain to be elucidated. Through GC-MS analysis we found that the majority of metabolites show oscillatory dynamics, with 70% of the identified metabolite concentrations peaking in conjunction with NAD(P)H. Through statistical analyses of microarray data, we identified that biosynthetic events have a defined order, and this program is initiated when respiration rates are increasing. We then combined metabolic, transcriptional data and statistical analyses of transcription factor activity, identified the top oscillatory parameters, and filtered a large-scale yeast interaction network according to these parameters. The analyses and controlled experimental perturbation provided evidence that a transcriptional complex formed part of the timing circuit for biosynthetic, reductive, and cell cycle programs in the cell. This circuitry does not act in isolation because both have strong translational, proteomic, and metabolic regulatory mechanisms. Our data lead us to conclude that the regulation of the respiratory oscillation revolves around coupled subgraphs containing large numbers of proteins and metabolites, with a potential to oscillate, and no definable hierarchy, i.e., heterarchical control. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Proceedings of the National Academy of Sciences of the United States of America en
dc.title Regulation of yeast oscillatory dynamics en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1073/pnas.0606677104
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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