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dc.contributor.author Munks, R. J. L.
dc.contributor.author Lehane, S. M.
dc.contributor.author Hamilton, Joanne V.
dc.contributor.author Lehane, Michael J.
dc.date.accessioned 2008-12-11T12:14:43Z
dc.date.available 2008-12-11T12:14:43Z
dc.date.issued 2008-12-11
dc.identifier.citation Munks , R J L , Lehane , S M , Hamilton , J V & Lehane , M J 2008 , ' Association of midgut defensin with a novel serine protease in the blood-sucking fly Stomoxys calcitrans ' Insect Molecular Biology , pp. 197-205 . en
dc.identifier.other PURE: 100678
dc.identifier.other dspace: 2160/1536
dc.identifier.uri http://hdl.handle.net/2160/1536
dc.identifier.uri http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2583.2002.00325.x en
dc.description J. V. Hamilton , R. J. L. Munks , S. M. Lehane and M. J. Lehane (2002). Association of midgut defensin with a novel serine protease in the blood-sucking fly Stomoxys calcitrans. Insect Molecular Biology, 11 (3) pp.197-205 en
dc.description.abstract Using ELISA we provide direct evidence that the midgut defensins of the blood-sucking fly Stomoxys calcitrans are secreted into the gut lumen. We show that midgut defensin peptide levels increase up to fortyfold in response to a blood meal but not to a sugar meal. The data suggests the midgut defensin genes are post-transcriptionally regulated and that their function is protection of the stored blood meal from bacterial attack while it awaits digestion. Using recombinant defensins produced in Pichia pastoris we demonstrate that while in the gut cells the midgut defensins are bound in an SDS-stable complex to proteins with an apparent molecular weight of > 26 kDa from which they are released when secreted into the gut lumen. This > 26 kDa protein (Ssp3) has been cloned and sequenced and is a member of the serine protease S1 family with homologies to multiple insect proteases and to vertebrate trypsins and elastases. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Insect Molecular Biology en
dc.title Association of midgut defensin with a novel serine protease in the blood-sucking fly Stomoxys calcitrans en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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