Show simple item record Skøt, Leif Humphreys, Janet Humphreys, Mervyn O. Thorogood, Daniel Gallagher, Joseph Anthony Sanderson, Ruth Armstead, Ian P. Thomas, Ian D. 2008-12-11T13:46:29Z 2008-12-11T13:46:29Z 2007-09-01
dc.identifier.citation Skøt , L , Humphreys , J , Humphreys , M O , Thorogood , D , Gallagher , J A , Sanderson , R , Armstead , I P & Thomas , I D 2007 , ' Association of candidate genes with flowering time and water-soluble carbohydrate content in Lolium perenne (L.) ' Genetics , vol 177 , no. 1 , pp. 535-547 . DOI: 10.1534/genetics.107.071522 en
dc.identifier.issn 1943-2631
dc.identifier.other PURE: 92245
dc.identifier.other PURE UUID: b9a43c0e-e39d-4ced-8f1d-04a89323faea
dc.identifier.other dspace: 2160/1547
dc.identifier.other DSpace_20121128.csv: row: 1240
dc.identifier.other Scopus: 34547741261
dc.identifier.other PubMed: 17660575
dc.identifier.other PubMedCentral: PMC2013705
dc.identifier.uri en
dc.description Skøt, L., Humphreys, J., Humphreys, M. O., Thorogood, D., Gallagher, J. A., Sanderson, R., Armstead, I. P., Thomas, I. D. (2007). Association of candidate genes with flowering time and water-soluble carbohydrate content in Lolium perenne (L.). Genetics, 177 (1), 535-547. Sponsorship: BBSRC RAE2008 en
dc.description.abstract We describe a candidate gene approach for associating SNPs with variation in flowering time and water-soluble carbohydrate (WSC) content and other quality traits in the temperate forage grass species Lolium perenne. Three analysis methods were used, which took the significant population structure into account. First, a linear mixed model was used enabling a structured association analysis to be incorporated with the nine populations identified in the structure analysis as random variables. Second, a within-population analysis of variance was performed. Third, a tree-scanning method was used, in which haplotype trees were associated with phenotypes on the basis of inferred haplotypes. Analysis of variance within populations identified several associations between WSC, nitrogen (N), and dry matter digestibility with allelic variants within an alkaline invertase candidate gene LpcAI. These associations were only detected in material harvested in one of the two years. By contrast, consistent associations between the L. perenne homolog (LpHD1) of the rice photoperiod control gene HD1 and flowering time were identified. One SNP, in the immediate upstream region of the LpHD1 coding sequence (C-4443-A), was significant in the linear mixed model. Within-population analysis of variance and tree-scanning analysis confirmed and extended this result to the 2118 polymorphisms in some of the populations. The merits of the tree-scanning method are compared to the single SNP analysis. The potential usefulness of the 4443 SNP in marker-assisted selection is currently being evaluated in test crosses of genotypes from this work with turf-grass varieties. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Genetics en
dc.rights en
dc.title Association of candidate genes with flowering time and water-soluble carbohydrate content in Lolium perenne (L.) en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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