Proteomic analysis of the larval stage of the parasite Echinococcus granulosus: causative agent of cystic hydatid disease

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dc.contributor.author Ferreira, Henrique B.
dc.contributor.author Chemale, Gustavo
dc.contributor.author Zaha, Arnaldo
dc.contributor.author van Rossum, Arjan J.
dc.contributor.author Brophy, Peter M.
dc.contributor.author Barrett, John
dc.contributor.author Jefferies, James R.
dc.date.accessioned 2008-12-11T14:03:14Z
dc.date.available 2008-12-11T14:03:14Z
dc.date.issued 2003-08-12
dc.identifier.citation Ferreira , H B , Chemale , G , Zaha , A , van Rossum , A J , Brophy , P M , Barrett , J & Jefferies , J R 2003 , ' Proteomic analysis of the larval stage of the parasite Echinococcus granulosus: causative agent of cystic hydatid disease ' Proteomics , pp. 1633-1636 . , 10.1002/pmic.200300487 en
dc.identifier.issn 1615-9861
dc.identifier.other PURE: 92375
dc.identifier.other dspace: 2160/1550
dc.identifier.uri http://hdl.handle.net/2160/1550
dc.identifier.uri http://www3.interscience.wiley.com/cgi-bin/abstract/104553341/ en
dc.description Gustavo Chemale, Arjan J. van Rossum, James R. Jefferies, John Barrett, Peter M. Brophy, Henrique B. Ferreira, Arnaldo Zaha (2003). Proteomic analysis of the larval stage of the parasite Echinococcus granulosus: causative agent of cystic hydatid disease. Proteomics, 3(8), 1633-1636. Sponsorship: CNPq / PADCT/CNPq / FAPERGS (Brazil)/ BBSRC (UK) RAE2008 en
dc.description.abstract We describe the preparation of Echinococcus granulosus metacestode protein extracts for two-dimensional electrophoresis (2-DE). Protoscoleces and hydatid fluid were prepared by precipitation using trichloroacetic acid (TCA) to remove nonprotein contaminants. Compared to the untreated control, TCA precipitation improved the 2-DE gel profile of the protoscoleces proteins. Comparison of 2-DE gels from insoluble and soluble fractions of the protoscoleces protein extract showed that most proteins are insoluble after lysis by sonication. Host serum proteins, especially albumin and globulins, caused horizontal streaking problems on the hydatid fluid 2-DE gels due to their high content in this sample. Even after the preparation of a hydatid fluid parasite enriched fraction, the high amount of bovine serum albumin and globulins made parasite-specific proteins difficult to detect by 2-DE. Despite the absence of an E. granulosus genome sequencing or expressed sequence tag (EST) projects, it was possible to identify 15 prominent protein spots from a whole protein protoscoleces 2-DE gel by peptide mass fingerprinting. These include actins, tropomyosin, paramyosin, thioredoxin reductase, antigen P-29, cyclophilin, and the heat shock proteins hsp70 and hsp20. This work demonstrates that 2-DE and PMF are important tools to identify proteins from the hydatid fluid and protoscoleces and for the comparative analysis of cysts from different hosts or between active and resting cysts. en
dc.format.extent 4 en
dc.language.iso eng
dc.relation.ispartof Proteomics en
dc.title Proteomic analysis of the larval stage of the parasite Echinococcus granulosus: causative agent of cystic hydatid disease en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1002/pmic.200300487
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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