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dc.contributor.author Hesketh, A. R.
dc.contributor.author Chandra, G.
dc.contributor.author Shaw, Adrian
dc.contributor.author Rowland, Jem
dc.contributor.author Kell, Douglas B.
dc.contributor.author Bibb, M. J.
dc.contributor.author Chater, K. F.
dc.date.accessioned 2006-04-25T15:49:36Z
dc.date.available 2006-04-25T15:49:36Z
dc.date.issued 2002
dc.identifier.citation Hesketh, A. R., Chandra, G., Shaw, A. D., Rowland, J. J., Kell, D. B., Bibb, M.J. & Chater, K. F. (2002). Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor. Mol. Microbiol. 46, 917-932 en
dc.identifier.uri http://hdl.handle.net/2160/155
dc.description.abstract The newly sequenced genome of Streptomyces coelicolor is estimated to encode 7825 theoretical proteins. We have mapped approximately 10% of the theoretical proteome experimentally using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Products from 770 different genes were identified, and the types of proteins represented are discussed in terms of their annotated functional classes. An average of 1.2 proteins per gene was observed, indicating extensive post-translational regulation. Examples of modification by N-acetylation, adenylylation and proteolytic processing were characterized using mass spectrometry. Proteins from both primary and certain secondary metabolic pathways are strongly represented on the map, and a number of these enzymes were identified at more than one two-dimensional gel location. Post-translational modification mechanisms may therefore play a significant role in the regulation of these pathways. Unexpectedly, one of the enzymes for synthesis of the actinorhodin polyketide antibiotic appears to be located outside the cytoplasmic compartment, within the cell wall matrix. Of 20 gene clusters encoding enzymes characteristic of secondary metabolism, eight are represented on the proteome map, including three that specify the production of novel metabolites. This information will be valuable in the characterization of the new metabolites. en
dc.language.iso en en
dc.relation.isreferencedby http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2002.03219.x en
dc.title Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor en
dc.type Text en
dc.type.publicationtype refereed published journal paper en
dc.identifier.duplicate True


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