Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry

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dc.contributor.author Day, Jennifer Penelope
dc.contributor.author Griffith, Gareth W.
dc.contributor.author Kell, Douglas B.
dc.date.accessioned 2008-12-11T16:58:00Z
dc.date.available 2008-12-11T16:58:00Z
dc.date.issued 2008-12-11
dc.identifier.citation Day , J P , Griffith , G W & Kell , D B 2008 , ' Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry ' Applied and Environmental Microbiology , pp. 37-45 . en
dc.identifier.other PURE: 101302
dc.identifier.other dspace: 2160/1564
dc.identifier.uri http://hdl.handle.net/2160/1564
dc.identifier.uri http://aem.asm.org/cgi/reprint/68/1/37 en
dc.description Jennifer P. Day, Douglas B. Kell, and Gareth W. Griffith (2002). Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry. Applied and Environmental Microbiology, 68(1), 37-45. en
dc.description.abstract The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or Botrytis spp., rust urediniospores, and pollen of grasses and plantain. Differentiation between P. infestans sporangia and powdery mildew conidia was not possible by these two methods but, when combined with analytical rules evolved by genetic programming methods, could be achieved after staining with the fluorescent brightener Calcofluor white M2R. The potential application of these techniques to the prediction of late-blight epiphytotics in the field is discussed. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Applied and Environmental Microbiology en
dc.title Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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