Show simple item record Roca, Maria James, Caron L. Pruzinská, Adriana Hörtensteiner, Stefan Thomas, Howard Ougham, Helen J. 2008-12-12T11:31:14Z 2008-12-12T11:31:14Z 2004-05-01
dc.identifier.citation Roca , M , James , C L , Pruzinská , A , Hörtensteiner , S , Thomas , H & Ougham , H J 2004 , ' Analysis of the chlorophyll catabolism pathway in leaves of an introgression senescence mutant of Lolium temulentum ' Phytochemistry , vol 65 , no. 9 , pp. 1231-1238 . DOI: 10.1016/j.phytochem.2004.03.022 en
dc.identifier.issn 1873-3700
dc.identifier.other PURE: 92705
dc.identifier.other PURE UUID: 1cdb80d2-f863-4505-b240-9df65e0367b6
dc.identifier.other dspace: 2160/1571
dc.identifier.other DSpace_20121128.csv: row: 1255
dc.identifier.other Scopus: 2942622113
dc.description Maria Roca, Caron James, Adriana Pruzinská, Stefan Hörtensteiner, Howard Thomas and Helen Ougham. Analysis of the chlorophyll catabolism pathway in leaves of an introgression senescence mutant of Lolium temulentum. Phytochemistry, 65 (9), 1231-1238. Sponsorship: BBSRC RAE2008 en
dc.description.abstract Pigments, proteins and enzyme activity related to chlorophyll catabolism were analysed in senescing leaves of wild-type (WT) Lolium temulentum and compared with those of an introgression line carrying a mutant gene from stay-green (SG) Festuca pratensis. During senescence of WT leaves chlorophylls a and b were continuously catabolised to colourless products and no other derivatives were observed, whereas in SG leaves there was an accumulation of dephytylated and oxidised catabolites including chlorophyllide a, phaeophorbide a and 132 OH-chlorophyllide a. Dephytylated products were absent from SG leaf tissue senescing under a light-dark cycle. Retention of pigments in SG was accompanied by significant stabilisation of light harvesting chlorophyll-proteins compared with WT, but soluble proteins such as Rubisco were degraded during senescence at a similar rate in the two genotypes. The activity of phaeophorbide a oxygenase measured in SG tissue at 3d was less than 12% of that in WT tissue at the same time-point during senescence and of the same order as that in young pre-senescent WT leaves, indicating that the metabolic lesion in SG concerns a deficiency at the ring-opening step of the catabolic pathway. In senescent L. temulentum tissue two terminal chlorophyll catabolites were identified with chromatographic characteristics that suggest they may represent hitherto undescribed catabolite structures. These data are discussed in relation to current understanding of the genetic and metabolic control of chlorophyll catabolism in leaf senescence. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Phytochemistry en
dc.rights en
dc.subject Lolium temulentum en
dc.subject Festuca pratensis en
dc.subject poaceae en
dc.subject catabolism en
dc.subject senescence en
dc.subject chlorophyll en
dc.subject introgression en
dc.subject mutant en
dc.subject chlorophyllide en
dc.subject phaeophorbide en
dc.subject phaeophorbide a oxygenase en
dc.title Analysis of the chlorophyll catabolism pathway in leaves of an introgression senescence mutant of Lolium temulentum en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Cadair

Advanced Search