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dc.contributor.author Donnison, Iain S.
dc.contributor.author King, Ian P.
dc.contributor.author Wolny, Elzbieta
dc.contributor.author Jenkins, Glyn M.
dc.contributor.author Marasek, Agnieszka
dc.contributor.author Armstead, Ian P.
dc.contributor.author Hasterok, Robert
dc.contributor.author Idziak, Dominika
dc.contributor.author Draper, John H.
dc.contributor.author Thomas, Ann M.
dc.date.accessioned 2008-12-12T14:46:08Z
dc.date.available 2008-12-12T14:46:08Z
dc.date.issued 2006-05-01
dc.identifier.citation Donnison , I S , King , I P , Wolny , E , Jenkins , G M , Marasek , A , Armstead , I P , Hasterok , R , Idziak , D , Draper , J H & Thomas , A M 2006 , ' Alignment of the genomes of brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization ' Genetics , vol 173 , no. 1 , pp. 349-362 . , 10.1534/genetics.105.049726 en
dc.identifier.issn 1943-2631
dc.identifier.other PURE: 92840
dc.identifier.other dspace: 2160/1579
dc.identifier.uri http://hdl.handle.net/2160/1579
dc.identifier.uri http://www.genetics.org/cgi/content/abstract/173/1/349 en
dc.description Robert Hasterok, Agnieszka Marasek, Iain S. Donnison, Ian Armstead, Ann Thomas, Ian P. King, Elzbieta Wolny, Dominika Idziak, John Draper and Glyn Jenkins (2006). Alignment of the genomes of brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization.Genetics, 73 (1), 349-362. Sponsorship: Royal Society / BBSRC;BBSRC RAE2008 en
dc.description.abstract As part of an initiative to develop Brachypodium distachyon as a genomic 'bridge' species between rice and the temperate cereals and grasses, a BAC library has been constructed for the two diploid (2n = 2x = 10) genotypes, ABR1 and ABR5. The library consists of 9100 clones, with an approximate average insert size of 88 kb, representing 2.22 genome equivalents. To validate the usefulness of this species for comparative genomics and gene discovery in its larger genome relatives, the library was screened by PCR using primers designed on previously mapped rice and Poaceae sequences. Screening indicated a degree of synteny between these species and B. distachyon, which was confirmed by fluorescent in situ hybridization of the marker-selected BACs (BAC landing) to the 10 chromosome arms of the karyotype, with most of the BACs hybridizing as single loci on known chromosomes. Contiguous BACs colocalized on individual chromosomes, thereby confirming the conservation of genome synteny and proving that B. distachyon has utility as a temperate grass model species alternative to rice. en
dc.format.extent 14 en
dc.language.iso eng
dc.relation.ispartof Genetics en
dc.title Alignment of the genomes of brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1534/genetics.105.049726
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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