Protein kinase C regulation of cell spreading in the molluscan Biomphalaria glabrata embryonic (Bge) cell line

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dc.contributor.author Yoshino, Timothy P.
dc.contributor.author Humphries, Judith E.
dc.contributor.author Elizondo, Leah
dc.date.accessioned 2008-12-15T09:31:38Z
dc.date.available 2008-12-15T09:31:38Z
dc.date.issued 2001-09-26
dc.identifier.citation Yoshino , T P , Humphries , J E & Elizondo , L 2001 , ' Protein kinase C regulation of cell spreading in the molluscan Biomphalaria glabrata embryonic (Bge) cell line ' Biochimica et Biophysica Acta (BBA) - Molecular Cell Research , pp. 243-252 . en
dc.identifier.other PURE: 92948
dc.identifier.other dspace: 2160/1600
dc.identifier.uri http://hdl.handle.net/2160/1600
dc.identifier.uri http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T20-442RJNC-7&_user=10&_coverDate=09%2F26%2F2001&_rdoc=7&_fmt=summary&_orig=browse&_srch=doc-info(%23toc%234904%232001%23984599996%23266019%23FLA%23display%23Volume)&_cdi=4904&_sort=d&_docanchor=& en
dc.description Judith E. Humphries, Leah Elizondo and Timothy P. Yoshino (2001). Protein kinase C regulation of cell spreading in the molluscan Biomphalaria glabrata embryonic (Bge) cell line. Biochimica et Biophysica Acta - Molecular Cell Research, 1540(3), 243-252. Sponsorship: National Institutes of Health AI 15503 RAE2008 en
dc.description.abstract Cellular adhesion and spreading are critical components involved in the processes of cell and tissue development, and immune responses in molluscs, but at present, little is known regarding the signaling pathways involved in these basic cellular functions. In the present study, the molluscan Biomphalaria glabrata embryonic (Bge) cell line was used as an in vitro model to study the signal transduction pathways regulating molluscan cell adhesion and spreading behavior. Western blot analysis using antibodies specific to mitogen-activated protein kinase (MAPK) revealed the presence of an MAPK-like immunoreactive protein in Bge cells, that was phosphorylated upon exposure to phorbol myristate acetate (PMA). Moreover, Bge cell treatment with inhibitors of protein kinase C (PKC), Ras and MAPK kinase (Mek) suppressed PMA-induced expression of activated MAPK, suggesting that PKC-, Ras- and Mek-like molecules may be acting upstream of MAPK. Similarly, in vitro Bge cell-spreading assays were performed in conjunction with the same panel of inhibitors to determine the potential involvement of PKC, Ras and Mek in cellular adhesion/spreading. Results revealed a similar pattern of inhibition of cell-spreading behavior strongly implying that the Bge cell spreading also may be regulated through a MAPK-associated signal transduction pathway(s) involving proteins similar to PKC, Ras and Mek. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Biochimica et Biophysica Acta (BBA) - Molecular Cell Research en
dc.title Protein kinase C regulation of cell spreading in the molluscan Biomphalaria glabrata embryonic (Bge) cell line en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/S0167-4889(01)00136-7
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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