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dc.contributor.author Judith E. en_US
dc.contributor.author Michael J. en_US
dc.contributor.author Yi-Wen en_US
dc.contributor.author Walter en_US
dc.contributor.author Nikki J. en_US
dc.contributor.author Brett en_US
dc.contributor.author Pat en_US
dc.contributor.author Aaron G. en_US
dc.contributor.author Tim A. en_US
dc.date.accessioned 2008-12-15T09:37:18Z
dc.date.available 2008-12-15T09:37:18Z
dc.date.issued 2004-09-17 en_US
dc.identifier http://dx.doi.org/10.1074/jbc.M405624200 en_US
dc.identifier.citation Humphries , J E , Kimber , M J , Barton , Y-W , Hsu , W , Marks , N J , Greer , B , Harriott , P , Maule , A G & Day , T A 2004 , ' Structure and bioactivity of neuropeptide F from the human parasites Schistosoma mansoni and Schistosoma japonicum ' Journal of Biological Chemistry , vol 279 , pp. 39880-39885 . , 10.1074/jbc.M405624200 en_US
dc.identifier.other PURE: 92979 en_US
dc.identifier.other dspace: 2160/1602 en_US
dc.identifier.uri http://hdl.handle.net/2160/1602
dc.description.abstract The blood flukes Schistosoma mansoni and Schistosoma japonicum inflict immense suffering as agents of human schistosomiasis. Previous investigations have found the nervous systems of these worms contain abundant immunoreactivity to antisera targeting invertebrate neuropeptide Fs (NPFs) as well as structurally similar neuropeptides of the mammalian neuropeptide Y (NPY) family. Here, cDNAs encoding NPF in these worms were identified, and the mature neuropeptides from the two species differed by only a single amino acid. Both neuropeptides feature the characteristics common among NPFs; they are 36 amino acids long with a carboxyl-terminal Gly-Arg-X-Arg-Phe-amide and Tyr residues at positions 10 and 17 from the carboxyl terminus. Synthetic S. mansoni NPF potently inhibits the forskolin-stimulated accumulation of cAMP in worm homogenates, with significant effects at 10-11 M. This is the first demonstration of an endogenous inhibition of cAMP by an NPF, and because this is the predominant pathway associated with vertebrate NPY family peptides, it demonstrates a conservation of downstream signaling pathways used by NPFs and NPY peptides. en_US
dc.format.extent 6 en_US
dc.relation.ispartof Journal of Biological Chemistry en_US
dc.title Structure and bioactivity of neuropeptide F from the human parasites Schistosoma mansoni and Schistosoma japonicum en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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