Show simple item record Bablak, Pauline Jenkins, Glyn M. Ghosh-Biswas, Gadab C. Routledge, Andrew P. M. Mur, Luis A. J. Draper, John H. Hasterok, Robert 2008-12-15T11:41:53Z 2008-12-15T11:41:53Z 2001-12-06
dc.identifier.citation Bablak , P , Jenkins , G M , Ghosh-Biswas , G C , Routledge , A P M , Mur , L A J , Draper , J H & Hasterok , R 2001 , ' Brachypodium distachyon. A new model system for functional genomics in grasses ' Plant Physiology , pp. 1539-1555 . DOI: 10.1104/pp.010196 en
dc.identifier.issn 0032-0889
dc.identifier.other PURE: 93177
dc.identifier.other PURE UUID: f3c78ebc-d836-4465-a254-2765a7957e26
dc.identifier.other dspace: 2160/1611
dc.identifier.other DSpace_20121128.csv: row: 1271
dc.identifier.other Scopus: 0035543457
dc.identifier.uri en
dc.description John Draper, Luis A.J. Mur, Glyn Jenkins, Gadab C. Ghosh-Biswas, Pauline Bablak, Robert Hasterok,and Andrew P.M. Routledge (2001). Brachypodium distachyon. A new model system for functional genomics in grasses. Plant Physiology, 127 (4), 1539-1555. Sponsorship: BBSRC / Gatsby Foundation RAE2008 en
dc.description.abstract A new model for grass functional genomics is described based on Brachypodium distachyon, which in the evolution of the Pooideae diverged just prior to the clade of 'core pooid' genera that contain the majority of important temperate cereals and forage grasses. Diploid ecotypes of B. distachyon (2n = 10) have five easily distinguishable chromosomes that display high levels of chiasma formation at meiosis. The B. distachyon nuclear genome was indistinguishable in size from that of Arabidopsis, making it the simplest genome described in grasses to date. B. distachyon is a self-fertile, inbreeding annual with a life cycle of less than 4 months. These features, coupled with its small size (approximately 20 cm at maturity), lack of seed-head shatter, and undemanding growth requirements should make it amenable to high-throughput genetics and mutant screens. Immature embryos exhibited a high capacity for plant regeneration via somatic embryogenesis. Regenerated plants display very low levels of albinism and have normal fertility. A simple transformation system has been developed based on microprojectile bombardment of embryogenic callus and hygromycin selection. Selected B. distachyon ecotypes were resistant to all tested cereal-adapted Blumeria graminis species and cereal brown rusts (Puccinia reconditia). In contrast, different ecotypes displayed resistance or disease symptoms following challenge with the rice blast pathogen (Magnaporthe grisea) and wheat/barley yellow stripe rusts (Puccinia striformis). Despite its small stature, B. distachyon has large seeds that should prove useful for studies on grain filling. Such biological characteristics represent important traits for study in temperate cereals. en
dc.format.extent 17 en
dc.language.iso eng
dc.relation.ispartof Plant Physiology en
dc.rights en
dc.title Brachypodium distachyon. A new model system for functional genomics in grasses en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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