Show simple item record Pauline en_US Glyn M. en_US Gadab C. en_US Andrew P. M. en_US Luis A. J. en_US John H. en_US Robert en_US 2008-12-15T11:41:53Z 2008-12-15T11:41:53Z 2001-12-06 en_US
dc.identifier.citation Bablak , P , Jenkins , G M , Ghosh-Biswas , G C , Routledge , A P M , Mur , L A J , Draper , J H & Hasterok , R 2001 , ' Brachypodium distachyon. A new model system for functional genomics in grasses ' Plant Physiology , pp. 1539-1555 . en_US
dc.identifier.other PURE: 93177 en_US
dc.identifier.other dspace: 2160/1611 en_US
dc.description.abstract A new model for grass functional genomics is described based on Brachypodium distachyon, which in the evolution of the Pooideae diverged just prior to the clade of 'core pooid' genera that contain the majority of important temperate cereals and forage grasses. Diploid ecotypes of B. distachyon (2n = 10) have five easily distinguishable chromosomes that display high levels of chiasma formation at meiosis. The B. distachyon nuclear genome was indistinguishable in size from that of Arabidopsis, making it the simplest genome described in grasses to date. B. distachyon is a self-fertile, inbreeding annual with a life cycle of less than 4 months. These features, coupled with its small size (approximately 20 cm at maturity), lack of seed-head shatter, and undemanding growth requirements should make it amenable to high-throughput genetics and mutant screens. Immature embryos exhibited a high capacity for plant regeneration via somatic embryogenesis. Regenerated plants display very low levels of albinism and have normal fertility. A simple transformation system has been developed based on microprojectile bombardment of embryogenic callus and hygromycin selection. Selected B. distachyon ecotypes were resistant to all tested cereal-adapted Blumeria graminis species and cereal brown rusts (Puccinia reconditia). In contrast, different ecotypes displayed resistance or disease symptoms following challenge with the rice blast pathogen (Magnaporthe grisea) and wheat/barley yellow stripe rusts (Puccinia striformis). Despite its small stature, B. distachyon has large seeds that should prove useful for studies on grain filling. Such biological characteristics represent important traits for study in temperate cereals. en_US
dc.format.extent 17 en_US
dc.relation.ispartof Plant Physiology en_US
dc.title Brachypodium distachyon. A new model system for functional genomics in grasses en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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