Show simple item record Kaprelyants, Arseny S. Mukamolova, Galina V. Young, Michael Turapov, Obolbek A. Telkov, Miroslav Kazarian, Konstantin Kell, Douglas B. 2008-12-15T14:05:53Z 2008-12-15T14:05:53Z 2002-11-01
dc.identifier.citation Kaprelyants , A S , Mukamolova , G V , Young , M , Turapov , O A , Telkov , M , Kazarian , K & Kell , D B 2002 , ' The rpf gene of Micrococcus luteus encodes an essential secreted growth factor ' Molecular Microbiology , pp. 611-621 . , 10.1046/j.1365-2958.2002.03183.x en
dc.identifier.issn 1365-2958
dc.identifier.other PURE: 93301
dc.identifier.other dspace: 2160/1617
dc.identifier.uri en
dc.description Galina V. Mukamolova, Obolbek A. Turapov, Konstantin Kazarian, Miroslav Telkov, Arseny S. Kaprelyants, Douglas B. Kell and Michael Young (2002). The rpf gene of Micrococcus luteus encodes an essential secreted growth factor. Molecular Microbiology, 46 (3), 611-621. Sponsorship: BBSRC / Russian Foundation for Basic Research (grant 00-04-48691)/ WHO Global Programme for Vaccines and Immunization / Wellcome Trust RAE2008 en
dc.description.abstract Micrococcus luteus secretes a small protein called Rpf, which has autocrine and paracrine signalling functions and is required for the resuscitation of dormant cells. Originally isolated from the supernatant of actively growing cultures, Rpf was also detected on the surface of actively growing bacteria. Most molecules may be sequestered non-productively at the cell surface, as a truncated form of the protein, encompassing only the 'Rpf domain' is fully active. The C-terminal LysM module, which probably mediates binding to the cell envelope, is not required for biological activity. Rpf was essential for growth of M. luteus. Washed cells, inoculated at low density into a minimal medium, could not grow in its absence. Moreover, the incorporation of anti-Rpf antibodies into the culture medium at the time of inoculation also prevented bacterial growth. We were unable to inactivate rpf using a disrupted form of the gene, in which most of the coding sequence was replaced with a selectable thiostrepton resistance marker. Gene disruption was possible in the presence of a second, functional, plasmid-located copy of rpf, but not in the presence of a rpf derivative whose protein product lacked the secretory signal sequence. As far as we are aware, Rpf is the first example of a truly secreted protein that is essential for bacterial growth. If the Rpf-like proteins elaborated by Mycobacterium tuberculosis and other mycobacteria prove similarly essential, interference with their proper functioning may offer novel opportunities for protecting against, and treating, tuberculosis and other mycobacterial disease. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Molecular Microbiology en
dc.title The rpf gene of Micrococcus luteus encodes an essential secreted growth factor en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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