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dc.contributor.author Kaderbhai, Mustak A.
dc.contributor.author Hopper, David J.
dc.contributor.author Akhtar, Kalim M.
dc.contributor.author Abbas, Syed K.
dc.contributor.author Kaderbhai, Naheed N.
dc.date.accessioned 2008-12-15T14:19:43Z
dc.date.available 2008-12-15T14:19:43Z
dc.date.issued 2003-08-01
dc.identifier.citation Mustak A. Kaderbhai, David J. Hopper, Kalim M. Akhtar, Syed K. Abbas, and Naheed N. Kaderbhai (2003). A cytochrome c from a lupanine-transforming Pseudomonas putida strain is expressed in Escherichia coli during aerobic cultivation and efficiently exported and assembled in the periplasm. Applied and Environmental Microbiology, 69 (8), 4727-4731. en
dc.identifier.isbn 1098-5336 en_US
dc.identifier.uri http://hdl.handle.net/2160/1619
dc.description.abstract We have cloned, sequenced, and heterologously expressed a periplasmic cytochrome c from a lupanine-utilizing Pseudomonas putida strain. Aerobic batch cultivation of Escherichia coli TB1 harboring the cytochrome c gene placed downstream of the lac promoter in pUC9 vector resulted in significant production of the holo-cytochrome c in the periplasm (4 mg of hemoprotein/liter of culture). The recombinant cytochrome c was purified to homogeneity and was found to be functional in accepting electrons from lupanine hydroxylase while catalyzing hydroxylation of lupanine. Comparison of the N-terminal amino acid sequence of the isolated cytochrome c with that deduced from the DNA sequence indicated that the signal sequence was processed at the bond position predicted by the SigPep program. The molecular size of the cytochrome c determined by electrospray mass spectrometry (9,595) was in precise agreement with that predicted from the nucleotide sequence. en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.relation.ispartof RAE2008 en_US
dc.relation.isreferencedby http://dx.doi.org/10.1128/AEM.69.8.4727-4731.2003 en
dc.title A cytochrome c from a lupanine-transforming Pseudomonas putida strain is expressed in Escherichia coli during aerobic cultivation and efficiently exported and assembled in the periplasm en
dc.type Text en
dc.type.publicationtype refereed published journal paper en
dc.identifier.doi http://aem.asm.org/cgi/reprint/69/8/4727
dc.identifier.duplicate True


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