BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment

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dc.contributor.author Jenkins, Glyn M.
dc.contributor.author Hasterok, Robert
dc.date.accessioned 2008-12-16T14:04:27Z
dc.date.available 2008-12-16T14:04:27Z
dc.date.issued 2007-02-15
dc.identifier.citation Jenkins , G M & Hasterok , R 2007 , ' BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment ' Nature Protocols , vol 2 , pp. 88-98 . en
dc.identifier.issn 1750-2799
dc.identifier.other PURE: 93524
dc.identifier.other dspace: 2160/1709
dc.identifier.uri http://hdl.handle.net/2160/1709
dc.identifier.uri http://www.nature.com/nprot/journal/v2/n1/abs/nprot.2006.490.html en
dc.description Glyn Jenkins & Robert Hasterok (2007). BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment. Nature Protocols, 2(1), 88-98. RAE2008 en
dc.description.abstract Fluorescence in situ hybridization (FISH) using bacterial artificial chromosomes (BACs) with large genomic DNA inserts as probes (BAC 'landing') is a powerful means by which eukaryotic genomes can be physically mapped and compared. Here we report a BAC landing protocol that has been developed specifically for the weedy grass species Brachypodium distachyon, which has been adopted recently by the scientific community as an alternative model for the temperate cereals and grasses. The protocol describes the preparation of somatic and meiotic chromosome substrates for FISH, the labeling of BACs, a chromosome mapping strategy, empirical conditions for optimal in situ hybridization and stringency washing, the detection of probes and the capturing and processing of images. The expected outcome of the protocol is the specific assignment of BACs containing single-copy inserts to one of the five linkage groups of the genome of this species. Once somatic or meiotic material is available, the entire protocol can be completed in about 3 d. The protocol has been customized empirically for B. distachyon and its near relatives, but it can be adapted with minor modifications to diverse plant species. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Nature Protocols en
dc.title BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1038/nprot.2006.490
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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