Show simple item record Glyn M. en_US Robert en_US 2008-12-16T14:04:27Z 2008-12-16T14:04:27Z 2007-02-15 en_US
dc.identifier en_US
dc.identifier.citation Jenkins , G M & Hasterok , R 2007 , ' BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment ' Nature Protocols , vol 2 , pp. 88-98 . , 10.1038/nprot.2006.490 en_US
dc.identifier.other PURE: 93524 en_US
dc.identifier.other dspace: 2160/1709 en_US
dc.description.abstract Fluorescence in situ hybridization (FISH) using bacterial artificial chromosomes (BACs) with large genomic DNA inserts as probes (BAC 'landing') is a powerful means by which eukaryotic genomes can be physically mapped and compared. Here we report a BAC landing protocol that has been developed specifically for the weedy grass species Brachypodium distachyon, which has been adopted recently by the scientific community as an alternative model for the temperate cereals and grasses. The protocol describes the preparation of somatic and meiotic chromosome substrates for FISH, the labeling of BACs, a chromosome mapping strategy, empirical conditions for optimal in situ hybridization and stringency washing, the detection of probes and the capturing and processing of images. The expected outcome of the protocol is the specific assignment of BACs containing single-copy inserts to one of the five linkage groups of the genome of this species. Once somatic or meiotic material is available, the entire protocol can be completed in about 3 d. The protocol has been customized empirically for B. distachyon and its near relatives, but it can be adapted with minor modifications to diverse plant species. en_US
dc.format.extent 11 en_US
dc.relation.ispartof Nature Protocols en_US
dc.title BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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