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dc.contributor.author Gibson, W.
dc.contributor.author Lehane, Michael J.
dc.contributor.author Hamilton, Joanne V.
dc.contributor.author al, et
dc.contributor.author Kerhornou, A.
dc.contributor.author Berriman, M.
dc.contributor.author Aksoy, S.
dc.date.accessioned 2008-12-16T14:17:21Z
dc.date.available 2008-12-16T14:17:21Z
dc.date.issued 2008-12-16
dc.identifier.citation Gibson , W , Lehane , M J , Hamilton , J V , al , Kerhornou , A , Berriman , M & Aksoy , S 2008 , Adult midgut expressed sequence tags from the tsetse fly Glossina morsitans morsitans and expression analysis of putative immune response genes . BioMed Central Ltd . en
dc.identifier.isbn 1465-6914
dc.identifier.other PURE: 102357
dc.identifier.other dspace: 2160/1716
dc.identifier.uri http://hdl.handle.net/2160/1716
dc.identifier.uri http://genomebiology.com/2003/4/10/R63 en
dc.description M J Lehane1, S Aksoy, W Gibson, A Kerhornou, M Berriman, J Hamilton, M B Soares, M F Bonaldo, S Lehane and N Hall (2003). Adult midgut expressed sequence tags from the tsetse fly Glossina morsitans morsitans and expression analysis of putative immune response genes. Genome Biology, 4, R63. Open Access en
dc.description.abstract Background Tsetse flies transmit African trypanosomiasis leading to half a million cases annually. Trypanosomiasis in animals (nagana) remains a massive brake on African agricultural development. While trypanosome biology is widely studied, knowledge of tsetse flies is very limited, particularly at the molecular level. This is a serious impediment to investigations of tsetse-trypanosome interactions. We have undertaken an expressed sequence tag (EST) project on the adult tsetse midgut, the major organ system for establishment and early development of trypanosomes. Results A total of 21,427 ESTs were produced from the midgut of adult Glossina morsitans morsitans and grouped into 8,876 clusters or singletons potentially representing unique genes. Putative functions were ascribed to 4,035 of these by homology. Of these, a remarkable 3,884 had their most significant matches in the Drosophila protein database. We selected 68 genes with putative immune-related functions, macroarrayed them and determined their expression profiles following bacterial or trypanosome challenge. In both infections many genes are downregulated, suggesting a malaise response in the midgut. Trypanosome and bacterial challenge result in upregulation of different genes, suggesting that different recognition pathways are involved in the two responses. The most notable block of genes upregulated in response to trypanosome challenge are a series of Toll and Imd genes and a series of genes involved in oxidative stress responses. Conclusions The project increases the number of known Glossina genes by two orders of magnitude. Identification of putative immunity genes and their preliminary characterization provides a resource for the experimental dissection of tsetse-trypanosome interactions. en
dc.language.iso eng
dc.publisher BioMed Central Ltd
dc.title Adult midgut expressed sequence tags from the tsetse fly Glossina morsitans morsitans and expression analysis of putative immune response genes en
dc.type Text en
dc.type.publicationtype Report (commissioned) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en


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