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dc.contributor.author Dunne, David W.
dc.contributor.author Hoffmann, Karl F.
dc.contributor.author Johnston, David A.
dc.date.accessioned 2008-12-16T15:09:19Z
dc.date.available 2008-12-16T15:09:19Z
dc.date.issued 2008-12-16
dc.identifier.citation Dunne , D W , Hoffmann , K F & Johnston , D A 2008 , Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling . BioMed Central Ltd . en
dc.identifier.isbn 1465-6914
dc.identifier.other PURE: 102486
dc.identifier.other dspace: 2160/1725
dc.identifier.uri http://hdl.handle.net/2160/1725
dc.identifier.uri http://genomebiology.com/2002/3/8/research/0041 en
dc.description Karl F Hoffmann, David A Johnston and David W Dunne (2002). Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling. Genome Biology, 3(8):research0041. en
dc.description.abstract Background Parasitic helminths of the genus Schistosoma mate, achieve sexual maturity and produce eggs in the bloodstream of their definitive hosts, and the most important pathological consequences of the infection are associated with this process. We have used cDNA microarray technology to initiate genome-wide gene-expression studies of sex and sexual development in mature Schistosoma mansoni parasites. Results An S. mansoni-specific cDNA microarray was fabricated using 576 expressed sequence tags selected from three cDNA libraries and originating from two different parasite developmental stages. Five independent cDNA microarray hybridizations were analyzed using stringent filtering criteria and careful quality control, leading to the identification of 12 new female-associated and 4 new male-associated gene transcripts in the mature adult schistosome. Statistical analysis of variation demonstrated high levels of agreement within a cDNA microarray (correlation coefficient 0.91; median coefficient of variation 11.1%) and between cDNA microarrays (correlation coefficient 0.90; median coefficient of variation 14.4%). RT-PCR analysis confirmed the cDNA microarray results, thereby supporting the reliability of the system. Conclusions Our study expands the list of S. mansoni gender-associated gene transcripts from all previous studies by a factor of two. Among the new associations identified, a tyrosinase ortholog was preferentially expressed in the adult female, and a dynein light-chain ortholog was highly induced in the adult male. cDNA microarrays offer the potential for exponential leaps in the understanding of parasite biology and this study shows how molecules involved in sexual biology can be rapidly identified. en
dc.language.iso eng
dc.publisher BioMed Central Ltd
dc.title Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling en
dc.type Text en
dc.type.publicationtype Report (commissioned) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en


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