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dc.contributor.author Hirai, Hirohisa
dc.contributor.author Hoffmann, Karl F.
dc.contributor.author Hirai, Yuriko
dc.contributor.author Fitzpatrick, Jennifer M.
dc.date.accessioned 2008-12-16T15:55:14Z
dc.date.available 2008-12-16T15:55:14Z
dc.date.issued 2008-12-16
dc.identifier.citation Hirai , H , Hoffmann , K F , Hirai , Y & Fitzpatrick , J M 2008 , ' Schistosome egg production is dependent upon the activities of two developmentally regulated tyrosinases ' The FASEB Journal , pp. 823-835 . en
dc.identifier.other PURE: 102697
dc.identifier.other dspace: 2160/1732
dc.identifier.uri http://hdl.handle.net/2160/1732
dc.identifier.uri http://www.fasebj.org/cgi/content/full/21/3/823 en
dc.description Jennifer M. Fitzpatrick, Yuriko Hirai, Hirohisa Hirai and Karl F. Hoffmann (2007). Schistosome egg production is dependent upon the activities of two developmentally regulated tyrosinases. The FASEB Journal, 21 (7), 823-835. en
dc.description.abstract Egg production is responsible for life cycle progression and host immunopathology during schistosomiasis, with the associated parasite molecules being investigated as potential novel chemotherapeutic targets. Here, we characterize two Schistosoma mansoni products, tyrosinase 1 and tyrosinase 2 (SmTYR1/SmTYR2) and show that their diphenol oxidase enzyme activities are critical for eggshell formation and production. The genes encoding these bifunctional enzymes (monophenol and diphenol oxidases) result from a duplication event that likely occurred before speciation and exist in the parasite¿s genome as multiple copies, which are linked and localized to chromosomes 4 and W. SmTYR1/SmTYR2 transcription and diphenol oxidase action are developmentally regulated with most enzyme activity localized to the eggshell¿producing cells contained within the vitellaria of adult female worms. Importantly, kojic-acid mediated inhibition (IC50=0.5µM) of SmTYR1/SmTYR2¿s diphenol oxidase activity during in vitro culture of sexually mature adult worms resulted in a significant decrease in the production of phenotypically normal eggs. Therefore our data suggest that SmTYR1/2 inhibition represents a novel and potentially effective strategy for combating schistosomiasis and furthermore, it may point to new methods for combinatorial control of immunopathology and egg transmission during platyhelminth infection.¿Fitzpatrick J. M., Hirai Y., Hirai H., Hoffmann K. F. Schistosome egg production is dependent upon the activities of two developmentally regulated tyrosinases. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof The FASEB Journal en
dc.title Schistosome egg production is dependent upon the activities of two developmentally regulated tyrosinases en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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