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dc.contributor.author Nagai, Yasuhiro
dc.contributor.author Aso, Hisashi
dc.contributor.author Ogasawara, Hideki
dc.contributor.author Tanaka, Sachi
dc.contributor.author Taketa, Yoshikazu
dc.contributor.author Watanabe, Kouichi
dc.contributor.author Ohwada, Shyuichi
dc.contributor.author Rose, Michael T.
dc.contributor.author Kitazawa, Haruki
dc.contributor.author Yamaguchi, Takahiro
dc.date.accessioned 2008-12-19T15:22:35Z
dc.date.available 2008-12-19T15:22:35Z
dc.date.issued 2008-11-01
dc.identifier.citation Nagai , Y , Aso , H , Ogasawara , H , Tanaka , S , Taketa , Y , Watanabe , K , Ohwada , S , Rose , M T , Kitazawa , H & Yamaguchi , T 2008 , ' Anterior Pituitary Progenitor Cells Express Costimulatory Molecule 4Ig-B7-H31 ' Journal of Immunology , vol 181 , no. 9 , pp. 6073–6081 . en
dc.identifier.issn 0022-1767
dc.identifier.other PURE: 98001
dc.identifier.other dspace: 2160/1815
dc.identifier.uri http://hdl.handle.net/2160/1815
dc.identifier.uri http://www.ncbi.nlm.nih.gov/pubmed/18941196?dopt=Abstract en
dc.description Yasuhiro Nagai, Hisashi Aso, Hideki Ogasawara, Sachi Tanaka, Yoshikazu Taketa, Kouichi Watanabe, Shyuichi Ohwada, Michael T. Rose, Haruki Kitazawa, and Takahiro Yamaguchi (2008). Anterior pituitary progenitor cells express costimulatory molecule 4Ig-B7-H31. The Journal of Immunology, 181 (9), 6073–6081. en
dc.description.abstract Stem/Progenitor cells in the postnatal pituitary gland are embedded in a marginal cell layer around Rathke’s pouch. However, the nature and behavior of anterior pituitary progenitor cells remain unclear. We established bovine anterior pituitary progenitor cell line (BAPC)-1 from the anterior pituitary gland, which expressed stem/progenitor cell-related genes and several inflammatory cytokines. To characterize and localize these pituitary progenitor cells, we produced a mAb (12B mAb) against BAPC-1. The 12B mAb recognized the 4Ig-B7-H3 molecule, which is a costimulatory molecule and negative regulator in T cell activation. WC1 T cells in young bovine PBMC express the 4Ig-B7-H3 molecule, but few or no 4Ig-B7-H3-immunoreactive cells are expressed in PBMC in adult cattle. The 12B-immunoreactive cells in the bovine anterior pituitary gland were localized around Rathke’s pouch and expressed IL-18 and MHC class II. However, the number of 12B-immunoreactive cells was lower in adult than in young cattle. BAPC-1 expressed IL-18 and MHC class II, and demonstrated phagocytotic activity. BAPC-1 also had the ability to promote CD25 expression in PBMC after 5 days of coculture, and blocking 4Ig-B7-H3 12B mAb enhanced their expression of CD25. In addition, the 12B-immunoreactive cells were observed around the pars tuberalis closely bordering the median eminence and in the blood vessels of the primary portal plexus in the anterior pituitary gland. These results suggest that an established BAPC-1 may originate from these progenitor cells, and that the progenitor cells with 4Ig-B7-H3 may play a critical role in the immunoendocrine network. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Journal of Immunology en
dc.title Anterior Pituitary Progenitor Cells Express Costimulatory Molecule 4Ig-B7-H31 en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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