Show simple item record Nagai, Yasuhiro Aso, Hisashi Ogasawara, Hideki Tanaka, Sachi Taketa, Yoshikazu Watanabe, Kouichi Ohwada, Shyuichi Rose, Michael T. Kitazawa, Haruki Yamaguchi, Takahiro 2008-12-19T15:22:35Z 2008-12-19T15:22:35Z 2008-11-01
dc.identifier.citation Nagai , Y , Aso , H , Ogasawara , H , Tanaka , S , Taketa , Y , Watanabe , K , Ohwada , S , Rose , M T , Kitazawa , H & Yamaguchi , T 2008 , ' Anterior Pituitary Progenitor Cells Express Costimulatory Molecule 4Ig-B7-H31 ' Journal of Immunology , vol 181 , no. 9 , pp. 6073–6081 . en
dc.identifier.issn 0022-1767
dc.identifier.other PURE: 98001
dc.identifier.other PURE UUID: 83dce0ce-d6d2-4be8-965d-46f9c9724f6d
dc.identifier.other dspace: 2160/1815
dc.identifier.uri en
dc.description Yasuhiro Nagai, Hisashi Aso, Hideki Ogasawara, Sachi Tanaka, Yoshikazu Taketa, Kouichi Watanabe, Shyuichi Ohwada, Michael T. Rose, Haruki Kitazawa, and Takahiro Yamaguchi (2008). Anterior pituitary progenitor cells express costimulatory molecule 4Ig-B7-H31. The Journal of Immunology, 181 (9), 6073–6081. en
dc.description.abstract Stem/Progenitor cells in the postnatal pituitary gland are embedded in a marginal cell layer around Rathke’s pouch. However, the nature and behavior of anterior pituitary progenitor cells remain unclear. We established bovine anterior pituitary progenitor cell line (BAPC)-1 from the anterior pituitary gland, which expressed stem/progenitor cell-related genes and several inflammatory cytokines. To characterize and localize these pituitary progenitor cells, we produced a mAb (12B mAb) against BAPC-1. The 12B mAb recognized the 4Ig-B7-H3 molecule, which is a costimulatory molecule and negative regulator in T cell activation. WC1 T cells in young bovine PBMC express the 4Ig-B7-H3 molecule, but few or no 4Ig-B7-H3-immunoreactive cells are expressed in PBMC in adult cattle. The 12B-immunoreactive cells in the bovine anterior pituitary gland were localized around Rathke’s pouch and expressed IL-18 and MHC class II. However, the number of 12B-immunoreactive cells was lower in adult than in young cattle. BAPC-1 expressed IL-18 and MHC class II, and demonstrated phagocytotic activity. BAPC-1 also had the ability to promote CD25 expression in PBMC after 5 days of coculture, and blocking 4Ig-B7-H3 12B mAb enhanced their expression of CD25. In addition, the 12B-immunoreactive cells were observed around the pars tuberalis closely bordering the median eminence and in the blood vessels of the primary portal plexus in the anterior pituitary gland. These results suggest that an established BAPC-1 may originate from these progenitor cells, and that the progenitor cells with 4Ig-B7-H3 may play a critical role in the immunoendocrine network. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Journal of Immunology en
dc.rights en
dc.title Anterior Pituitary Progenitor Cells Express Costimulatory Molecule 4Ig-B7-H31 en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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