Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii

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dc.contributor.author Edwards, Joan E.
dc.contributor.author McEwan, Neil R.
dc.contributor.author Wallace, R. John
dc.date.accessioned 2009-05-21T08:08:58Z
dc.date.available 2009-05-21T08:08:58Z
dc.date.issued 2008-06-01
dc.identifier.citation Edwards , J E , McEwan , N R & Wallace , R J 2008 , ' Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii ' Journal of Applied Microbiology , vol 104 , no. 6 , pp. 1617-1623 . en
dc.identifier.issn 1365-2672
dc.identifier.other PURE: 101126
dc.identifier.other dspace: 2160/2261
dc.identifier.uri http://hdl.handle.net/2160/2261
dc.description Edwards, J. E., McEwan, N. R., Wallace, R. J. (2008). Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii.  Journal of Applied Microbiology, 104, (6), 1617-1623. On file IMPF: 02.50 RONO: 1920 3008 Sponsorship: SEERAD en
dc.description.abstract Aims: The recent EU ban of growth-promoting antibiotics in animal production was based on fears concerning antibiotic resistance being transmitted to human pathogens. This paper explores the adaptation mechanism of a common ruminal bacterium, Prevotella bryantii, to one of the banned compounds, flavomycin (flavophospholipol). Methods and Results: Growth in the presence of flavomycin (2 and 20 μg ml−1) was characterized by a concentration-dependent increase in the length of the lag phase, which decreased after previous flavomycin exposure. From growth patterns on solid medium, decreased sensitivity appeared to be due to a whole-population adaptation. Proteomic analysis indicated upregulation of three native proteins occurred following flavomycin adaptation. Further analysis of two of these proteins resulted in no database matches, suggesting that they may be species-specific. Flavomycin adaptation also resulted in co-adaptation to bacitracin and vancomycin. Conclusions: Adaptation of P. bryantii to flavomycin, which also resulted in co-adaptation to bacitracin and vancomycin, may involve an increased availability of undecaprenyl pyrophosphate. Significance and Impact of the Study: The use of flavomycin, and similar growth-promoting antibiotics, in animal production may prompt adaptive responses in ruminal bacteria which can significantly change their antibiotic sensitivity. en
dc.format.extent 7 en
dc.language.iso eng
dc.relation.ispartof Journal of Applied Microbiology en
dc.subject adaptation en
dc.subject antibiotic en
dc.subject flavomycin en
dc.subject Prevotella bryantii en
dc.subject resistance en
dc.subject rumen en
dc.title Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1111/j.1365-2672.2007.03689.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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