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dc.contributor.author Caligari, Peter D. S.
dc.contributor.author Rogers, Tom
dc.contributor.author Croxford, Adam E.
dc.contributor.author Wilkinson, Michael J.
dc.date.accessioned 2009-05-21T08:39:12Z
dc.date.available 2009-05-21T08:39:12Z
dc.date.issued 2008-08-05
dc.identifier.citation Caligari , P D S , Rogers , T , Croxford , A E & Wilkinson , M J 2008 , ' High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar ' New Phytologist , vol 180 , no. 3 , pp. 594-607 . , 10.1111/j.1469-8137.2008.02588.x , 10.1111/j.1469-8137.2008.02588.x en
dc.identifier.issn 1469-8137
dc.identifier.other PURE: 101247
dc.identifier.other dspace: 2160/2269
dc.identifier.uri http://hdl.handle.net/2160/2269
dc.description Croxford, A. E., Rogers, T., Caligari, P. D. S., Wilkinson, M. J. (2008). High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar.  New Phytologist, 180, (3), 594-607. IMPF: 05.18 RONO: 00 Sponsorship: DEFRA en
dc.description.abstract The provision of sequence-tagged site (STS) anchor points allows meaningful comparisons between mapping studies but can be a time-consuming process for nonmodel species or orphan crops. • Here, the first use of high-resolution melt analysis (HRM) to generate STS markers for use in linkage mapping is described. This strategy is rapid and low-cost, and circumvents the need for labelled primers or amplicon fractionation. • Using white lupin (Lupinus albus, x = 25) as a case study, HRM analysis was applied to identify 91 polymorphic markers from expressed sequence tag (EST)-derived and genomic libraries. Of these, 77 generated STS anchor points in the first fully resolved linkage map of the species. The map also included 230 amplified fragment length polymorphisms (AFLP) loci, spanned 1916 cM (84.2% coverage) and divided into the expected 25 linkage groups. • Quantitative trait loci (QTL) analyses performed on the population revealed genomic regions associated with several traits, including the agronomically important time to flowering (tf), alkaloid synthesis and stem height (Ph). Use of HRM-STS markers also allowed us to make direct comparisons between our map and that of the related crop, Lupinus angustifolius, based on the conversion of RFLP, microsatellite and single nucleotide polymorphism (SNP) markers into HRM markers. en
dc.format.extent 14 en
dc.language.iso eng
dc.relation.ispartof New Phytologist en
dc.title High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1111/j.1469-8137.2008.02588.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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