Show simple item record Marcia M. de O. en_US T. en_US B. D. en_US S. J. en_US Philip en_US 2009-05-21T08:57:14Z 2009-05-21T08:57:14Z 2008-04 en_US
dc.identifier en_US
dc.identifier.citation Buanafina , M M D O , Langdon , T , Hauck , B D , Dalton , S J & Morris , P 2008 , ' Expression of a fungal ferulic acid esterase increases cell wall digestibility of tall fescue (Festuca arundinacea) ' Plant Biotechnology Journal , vol 6 , no. 3 , pp. 264-280 . , 10.1111/j.1467-7652.2007.00317.x en_US
dc.identifier.other PURE: 101418 en_US
dc.identifier.other dspace: 2160/2273 en_US
dc.description.abstract In the cell walls of forage grasses, ferulic acid is esterified to arabinoxylans and participates with lignin monomers in oxidative coupling pathways to generate ferulate–polysaccharide–lignin complexes that cross-link the cell wall. Such cross-links hinder cell wall degradation by ruminant microbes, reducing plant digestibility. In this study, genetically modified Festuca arundinacea plants were produced expressing an Aspergillus niger ferulic acid esterase (FAEA) targeted to the vacuole. The rice actin promoter proved to be effective for FAEA expression, as did the cauliflower mosaic virus (CaMV) 35S and maize ubiquitin promoters. Higher levels of expression were, however, found with inducible heat-shock and senescence promoters. Following cell death and subsequent incubation, vacuole-targeted FAEA resulted in the release of both monomeric and dimeric ferulic acids from the cell walls, and this was enhanced several fold by the addition of exogenous endo-1,4-β-xylanase. Most of the FAEA-expressing plants showed increased digestibility and reduced levels of cell wall esterified phenolics relative to non-transformed plants. It is concluded that targeted FAEA expression is an effective strategy for improving wall digestibility in Festuca and, potentially, other grass species used for fodder or cellulosic ethanol production. en_US
dc.format.extent 17 en_US
dc.relation.ispartof Plant Biotechnology Journal en_US
dc.subject cell wall en_US
dc.subject digestibility en_US
dc.subject ferulic acid esterase en_US
dc.subject forage grass en_US
dc.subject hydroxycinnamic acids en_US
dc.subject transgenic plant en_US
dc.title Expression of a fungal ferulic acid esterase increases cell wall digestibility of tall fescue (Festuca arundinacea) en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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