Show simple item record Buanafina, Marcia M. de O. Langdon, T. Hauck, B. D. Dalton, S. J. Morris, Philip 2009-05-21T08:57:14Z 2009-05-21T08:57:14Z 2008-04
dc.identifier.citation Buanafina , M M D O , Langdon , T , Hauck , B D , Dalton , S J & Morris , P 2008 , ' Expression of a fungal ferulic acid esterase increases cell wall digestibility of tall fescue (Festuca arundinacea) ' Plant Biotechnology Journal , vol 6 , no. 3 , pp. 264-280 . DOI: 10.1111/j.1467-7652.2007.00317.x en
dc.identifier.issn 1467-7644
dc.identifier.other PURE: 101418
dc.identifier.other PURE UUID: b5701c63-96f8-4638-9fb2-e8a46b74baf8
dc.identifier.other dspace: 2160/2273
dc.identifier.other DSpace_20121128.csv: row: 1648
dc.identifier.other RAD: 2766
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 1380
dc.identifier.other IBERS: 0000018332
dc.identifier.other Ibers_20121112_1204.csv: row: 33
dc.identifier.other Scopus: 40349089733
dc.description Buanafina, M. M. de O., Langdon, T., Hauck, B., Dalton, S. J., Morris, P. (2008). Expression of a fungal ferulic acid esterase increases cell wall digestibility of tall fescue (Festuca arundinacea).  Plant Biotechnology Journal, 6 (3), 264-280. IMPF: 04.42 RONO: 1910 3007;1910 6729 Sponsorship: BBSRC en
dc.description.abstract In the cell walls of forage grasses, ferulic acid is esterified to arabinoxylans and participates with lignin monomers in oxidative coupling pathways to generate ferulate–polysaccharide–lignin complexes that cross-link the cell wall. Such cross-links hinder cell wall degradation by ruminant microbes, reducing plant digestibility. In this study, genetically modified Festuca arundinacea plants were produced expressing an Aspergillus niger ferulic acid esterase (FAEA) targeted to the vacuole. The rice actin promoter proved to be effective for FAEA expression, as did the cauliflower mosaic virus (CaMV) 35S and maize ubiquitin promoters. Higher levels of expression were, however, found with inducible heat-shock and senescence promoters. Following cell death and subsequent incubation, vacuole-targeted FAEA resulted in the release of both monomeric and dimeric ferulic acids from the cell walls, and this was enhanced several fold by the addition of exogenous endo-1,4-β-xylanase. Most of the FAEA-expressing plants showed increased digestibility and reduced levels of cell wall esterified phenolics relative to non-transformed plants. It is concluded that targeted FAEA expression is an effective strategy for improving wall digestibility in Festuca and, potentially, other grass species used for fodder or cellulosic ethanol production. en
dc.format.extent 17 en
dc.language.iso eng
dc.relation.ispartof Plant Biotechnology Journal en
dc.rights en
dc.subject cell wall en
dc.subject digestibility en
dc.subject ferulic acid esterase en
dc.subject forage grass en
dc.subject hydroxycinnamic acids en
dc.subject transgenic plant en
dc.title Expression of a fungal ferulic acid esterase increases cell wall digestibility of tall fescue (Festuca arundinacea) en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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