Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors

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dc.contributor.author Cairns, Andrew J.
dc.contributor.author Turner, Lesley B.
dc.contributor.author Gallagher, Joseph Anthony
dc.date.accessioned 2009-05-21T09:19:55Z
dc.date.available 2009-05-21T09:19:55Z
dc.date.issued 2008-09-17
dc.identifier.citation Cairns , A J , Turner , L B & Gallagher , J A 2008 , ' Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors ' New Phytologist , vol 180 , no. 4 , pp. 832-840 . en
dc.identifier.issn 1469-8137
dc.identifier.other PURE: 101515
dc.identifier.other dspace: 2160/2281
dc.identifier.uri http://hdl.handle.net/2160/2281
dc.description Cairns, A. J., Turner, L. B., Gallagher, J. A. (2008). Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors.  New Phytologist, 180, (4), 832-840. IMPF: 05.18 RONO: 1910 3007 en
dc.description.abstract Valid models are the foundation of systems biology. However, even well-established models may warrant reassessment. A testable feature of the currently accepted vacuolar model for fructan biosynthesis is its independence from metabolic energy at substrate level. The effects of limiting energy provision on fructan biosynthesis in grass leaves were determined. It was found that, in darkness in air, the rate of fructan accumulation was reduced to half relative to a light control. In darkness under anoxia the process was immediately abolished. In the light, the leaf sucrose concentration remained high, but in darkness ± O2, 40% of this sucrose was rapidly degraded. The constant rate of dark-aerobic fructan accumulation was independent of the decrease in sucrose concentration. Constant rates of aerobic fructan synthesis were independent of marked changes in extractable polymerase rates. In the dark under anoxia, fructan accumulation was abolished but leaves maintained ≥ 80% of the extractable polymerase. Extractable polymerase rates cannot explain the rates of fructan accumulation observed in vivo, if the process is vacuolar. It was shown that the results were inconsistent with a vacuolar site for fructan synthesis. Six inhibitors of endomembrane function were shown to abolish fructan synthesis in vivo en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof New Phytologist en
dc.subject anoxia en
dc.subject golgi en
dc.subject Lolium temulentum en
dc.subject monensin en
dc.subject respiration en
dc.title Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1111/j.1469-8137.2008.02616.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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