Show simple item record Andrew J. en_US Lesley B. en_US Joseph Anthony en_US 2009-05-21T09:19:55Z 2009-05-21T09:19:55Z 2008-09-17 en_US
dc.identifier en_US
dc.identifier.citation Cairns , A J , Turner , L B & Gallagher , J A 2008 , ' Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors ' New Phytologist , vol 180 , no. 4 , pp. 832-840 . , 10.1111/j.1469-8137.2008.02616.x en_US
dc.identifier.other PURE: 101515 en_US
dc.identifier.other dspace: 2160/2281 en_US
dc.description.abstract Valid models are the foundation of systems biology. However, even well-established models may warrant reassessment. A testable feature of the currently accepted vacuolar model for fructan biosynthesis is its independence from metabolic energy at substrate level. The effects of limiting energy provision on fructan biosynthesis in grass leaves were determined. It was found that, in darkness in air, the rate of fructan accumulation was reduced to half relative to a light control. In darkness under anoxia the process was immediately abolished. In the light, the leaf sucrose concentration remained high, but in darkness ± O2, 40% of this sucrose was rapidly degraded. The constant rate of dark-aerobic fructan accumulation was independent of the decrease in sucrose concentration. Constant rates of aerobic fructan synthesis were independent of marked changes in extractable polymerase rates. In the dark under anoxia, fructan accumulation was abolished but leaves maintained ≥ 80% of the extractable polymerase. Extractable polymerase rates cannot explain the rates of fructan accumulation observed in vivo, if the process is vacuolar. It was shown that the results were inconsistent with a vacuolar site for fructan synthesis. Six inhibitors of endomembrane function were shown to abolish fructan synthesis in vivo en_US
dc.format.extent 9 en_US
dc.relation.ispartof New Phytologist en_US
dc.subject anoxia en_US
dc.subject golgi en_US
dc.subject Lolium temulentum en_US
dc.subject monensin en_US
dc.subject respiration en_US
dc.title Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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