Show simple item record Cairns, Andrew J. Turner, Lesley B. Gallagher, Joseph Anthony 2009-05-21T09:19:55Z 2009-05-21T09:19:55Z 2008-12-01
dc.identifier.citation Cairns , A J , Turner , L B & Gallagher , J A 2008 , ' Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors ' New Phytologist , vol 180 , no. 4 , pp. 832-840 . DOI: 10.1111/j.1469-8137.2008.02616.x en
dc.identifier.issn 0028-646X
dc.identifier.other PURE: 101515
dc.identifier.other PURE UUID: ac65632b-39e9-4d65-9af8-04eb0a6f05c8
dc.identifier.other dspace: 2160/2281
dc.identifier.other DSpace_20121128.csv: row: 1651
dc.identifier.other IBERS: 0000017400
dc.identifier.other Ibers_20121112_1204.csv: row: 36
dc.identifier.other RAD: 2704
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 1366
dc.identifier.other Scopus: 55649100363
dc.description Cairns, A. J., Turner, L. B., Gallagher, J. A. (2008). Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors.  New Phytologist, 180, (4), 832-840. IMPF: 05.18 RONO: 1910 3007 en
dc.description.abstract Valid models are the foundation of systems biology. However, even well-established models may warrant reassessment. A testable feature of the currently accepted vacuolar model for fructan biosynthesis is its independence from metabolic energy at substrate level. The effects of limiting energy provision on fructan biosynthesis in grass leaves were determined. It was found that, in darkness in air, the rate of fructan accumulation was reduced to half relative to a light control. In darkness under anoxia the process was immediately abolished. In the light, the leaf sucrose concentration remained high, but in darkness ± O2, 40% of this sucrose was rapidly degraded. The constant rate of dark-aerobic fructan accumulation was independent of the decrease in sucrose concentration. Constant rates of aerobic fructan synthesis were independent of marked changes in extractable polymerase rates. In the dark under anoxia, fructan accumulation was abolished but leaves maintained ≥ 80% of the extractable polymerase. Extractable polymerase rates cannot explain the rates of fructan accumulation observed in vivo, if the process is vacuolar. It was shown that the results were inconsistent with a vacuolar site for fructan synthesis. Six inhibitors of endomembrane function were shown to abolish fructan synthesis in vivo en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof New Phytologist en
dc.rights en
dc.subject anoxia en
dc.subject golgi en
dc.subject Lolium temulentum en
dc.subject monensin en
dc.subject respiration en
dc.title Ryegrass leaf fructan synthesis is oxygen dependent and abolished by endomembrane inhibitors en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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