Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass

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dc.contributor.author Yoshida, Midori
dc.contributor.author Kanazawa, Akira
dc.contributor.author Yamada, Toshihiko
dc.contributor.author Kitamura, Keisuke
dc.contributor.author Hisano, Hiroshi
dc.contributor.author Iizuka, Masaru
dc.contributor.author Humphreys, Mervyn O.
dc.date.accessioned 2009-05-21T10:19:43Z
dc.date.available 2009-05-21T10:19:43Z
dc.date.issued 2008-03-13
dc.identifier.citation Yoshida , M , Kanazawa , A , Yamada , T , Kitamura , K , Hisano , H , Iizuka , M & Humphreys , M O 2008 , ' Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass ' New Phytologist , vol 178 , no. 4 , pp. 766-780 . en
dc.identifier.issn 1469-8137
dc.identifier.other PURE: 101837
dc.identifier.other dspace: 2160/2288
dc.identifier.uri http://hdl.handle.net/2160/2288
dc.description Hisano, H., Kanazawa, A., Yoshida, M., Humphreys, M. O., Iizuka, M., Kitamura, K., Yamada, T. (2008). Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass.  New Phytologist, 178, (4), 766-780. IMPF: 05.18 RONO: 1910 3007 Sponsorship: Ministry of Education, Culture, Sports, Science and Technology of Japan en
dc.description.abstract Fructan is the major nonstructural carbohydrate reserve in temperate grasses. To understand regulatory mechanisms in fructan synthesis and adaptation to cold environments, the isolation, functional characterization and genetic mapping of fructosyltransferase (FT) genes in perennial ryegrass (Lolium perenne) are described. • Six cDNAs (prft1–prft6) encoding FTs were isolated from cold-treated ryegrass plants, and three were positioned on a perennial ryegrass linkage map. Recombinant proteins were produced in Pichia pastoris and enzymatic activity was characterized. Changes in carbohydrate levels and mRNA levels of FT genes during cold treatment were also analysed. • One gene encodes sucrose-sucrose 1-fructosyltransferase (1-SST), and two gene encode fructan-fructan 6G-fructosyltransferase (6G-FFT). Protein sequences for the other genes (prfts 1, 2 and 6) were similar to sucrose-fructan 6-fructosyltransferase (6-SFT). The 1-SST and prft1 genes were colocalized with an invertase gene on the ryegrass linkage map. The mRNA levels of prft1 and prft2 increased gradually during cold treatment, while those of the 1-SST and 6G-FFT genes first increased, but then decreased before increasing again during a longer period of cold treatment. • Thus at least two different patterns of gene expression have developed during the evolution of functionally diverse FT genes, which are associated in a coordinated way with fructan synthesis in a cold environment. en
dc.format.extent 15 en
dc.language.iso eng
dc.relation.ispartof New Phytologist en
dc.title Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1111/j.1469-8137.2008.02409.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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