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dc.contributor.author Duval, Emilie
dc.contributor.author Parker, David
dc.contributor.author Enot, David P.
dc.contributor.author Draper, John H.
dc.contributor.author Beckmann, Manfred
dc.date.accessioned 2009-05-21T10:49:50Z
dc.date.available 2009-05-21T10:49:50Z
dc.date.issued 2009-05-21
dc.identifier.citation Duval , E , Parker , D , Enot , D P , Draper , J H & Beckmann , M 2009 , ' High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry ' Nature Protocols , pp. 486-504 . en
dc.identifier.other PURE: 110815
dc.identifier.other dspace: 2160/2292
dc.identifier.uri http://hdl.handle.net/2160/2292
dc.description Beckmann, M., Parker, D., Enot, D. P., Duval, E., Draper, J. (2008). High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry. Nature Protocols, 3, (3), pp. 486-504 en
dc.description.abstract Flow injection electrospray¿mass spectrometry (FIE¿MS) is finding utility as a first-pass metabolite fingerprinting tool in many research fields. We provide a protocol that has proved reliable in large-scale research projects involving diverse sample matrices originating from plants, microbes and mammalian biofluids. Using Brachypodium leaf material as an example matrix all steps are summarized from sample extraction to data quality assessment. Alternative procedures for dealing with other common matrices such as bloods and urine are included. With little sample pretreatment, no chromatography and instrument cycle times of 1,000 samples per week. Analysis of a typical batch of 240 samples (including first-pass data analysis) can be accomplished within 48 h. en
dc.format.extent 19 en
dc.language.iso eng
dc.relation.ispartof Nature Protocols en
dc.title High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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