Nitric oxide generation in Vicia faba phloem cells reveals them to be sensitive detectors as well as possible systemic transducers of stress

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dc.contributor.author Gaupels, Frank
dc.contributor.author Furch, Alexandra C. U.
dc.contributor.author Will, Torsten
dc.contributor.author Mur, Luis Alejandro Jose
dc.contributor.author Kogel, Karl-Heinz
dc.contributor.author Van Bel, Aart J. E.
dc.date.accessioned 2009-05-21T10:54:43Z
dc.date.available 2009-05-21T10:54:43Z
dc.date.issued 2008-05-01
dc.identifier.citation Gaupels , F , Furch , A C U , Will , T , Mur , L A J , Kogel , K-H & Van Bel , A J E 2008 , ' Nitric oxide generation in Vicia faba phloem cells reveals them to be sensitive detectors as well as possible systemic transducers of stress ' New Phytologist , vol 178 , no. 3 , pp. 634-646 . en
dc.identifier.issn 1469-8137
dc.identifier.other PURE: 102029
dc.identifier.other dspace: 2160/2295
dc.identifier.uri http://hdl.handle.net/2160/2295
dc.description Gaupels, F., Furch, A. C. U., Will, T., Mur, L. A. J., Kogel, K. H., van Bel, A. J. E. (2008). Nitric oxide generation in Vicia faba phloem cells reveals them to be sensitive detectors as well as possible systemic transducers of stress  New Phytologist, 178, (3), 634-646 On file IMPF: 05.18 RONO: 00 en
dc.description.abstract Vascular tissue was recently shown to be capable of producing nitric oxide (NO), but the production sites and sources were not precisely determined. Here, NO synthesis was analysed in the phloem of Vicia faba in response to stress- and pathogen defence-related compounds. The chemical stimuli were added to shallow paradermal cortical cuts in the main veins of leaves attached to intact plants. NO production in the bare-lying phloem area was visualized by real-time confocal laser scanning microscopy using the NO-specific fluorochrome 4,5-diaminofluorescein diacetate (DAF-2 DA). Abundant NO generation in companion cells was induced by 500 microm salicylic acid (SA) and 10 microm hydrogen peroxide (H(2)O(2)), but the fungal elicitor chitooctaose was much less effective. Phloem NO production was found to be dependent on Ca(2+) and mitochondrial electron transport and pharmacological approaches found evidence for activity of a plant NO synthase but not a nitrate reductase. DAF fluorescence increased most strongly in companion cells and was occasionally observed in phloem parenchyma cells. Significantly, accumulation of NO in sieve elements could be demonstrated. These findings suggest that the phloem perceives and produces stress-related signals and that one mechanism of distal signalling involves the production and transport of NO in the phloem. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof New Phytologist en
dc.subject hydrogen peroxide (H2O2) en
dc.subject nitric oxide synthase en
dc.subject nitric oxide (NO) en
dc.subject pathogen resistance en
dc.subject phloem en
dc.subject salicylic acid en
dc.subject stress en
dc.subject systemic signalling en
dc.title Nitric oxide generation in Vicia faba phloem cells reveals them to be sensitive detectors as well as possible systemic transducers of stress en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1111/j.1469-8137.2008.02388.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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