A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans

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dc.contributor.author Lacourse, James E.
dc.contributor.author Perally, Samirah
dc.contributor.author Hernandez-Viadel, Mariluz
dc.contributor.author Wright, Hazel A.
dc.contributor.author Brophy, Peter M.
dc.date.accessioned 2009-05-21T11:27:05Z
dc.date.available 2009-05-21T11:27:05Z
dc.date.issued 2008-06-27
dc.identifier.citation Lacourse , J E , Perally , S , Hernandez-Viadel , M , Wright , H A & Brophy , P M 2008 , ' A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans ' Journal of Proteome Research , vol 7 , no. 8 , pp. 3314-3318 . , 10.1021/pr8001035 en
dc.identifier.issn 1535-3893
dc.identifier.other PURE: 102482
dc.identifier.other dspace: 2160/2312
dc.identifier.uri http://hdl.handle.net/2160/2312
dc.identifier.uri http://pubs.acs.org/doi/pdf/10.1021/pr8001035 en
dc.description LaCourse, E. J., Perally, S., Hernandez-Viadel, M. Wright, H. A., Brophy, P. M. (2008). A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans. Journal of Proteome Research, 7, (8), pp. 3314-3318 Keywords: RNAi • Glutathione transferase • Caenorhabditis elegans C. elegans is a functional genomics model for studying related parasitic nematodes of veterinary importance. The paper showed the for the first time the application of proteomics to validate the outcome of RNAi knockdown in the model (Prot.RNAi), and showed for the first time evidence of genetic buffering ) in a protein superfamily (increased expression of family member on knockdown of another member). The major contributor to experimental design and manuscript write-up and NERC grant holder/PhD supervisor (JLaC) at Aberystwyth. On file IMPF: 05.68 en
dc.description.abstract Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations, protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans en
dc.format.extent 5 en
dc.language.iso eng
dc.relation.ispartof Journal of Proteome Research en
dc.subject RNAi en
dc.subject Glutathione transferase en
dc.subject Caenorhabditis elegans en
dc.title A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1021/pr8001035
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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