Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage

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dc.contributor.author Kanaya, Takashi
dc.contributor.author Miyazawa, Kohtaro
dc.contributor.author Takakura, Ikuro
dc.contributor.author Itani, Wataru
dc.contributor.author Watanabe, Kouichi
dc.contributor.author Ohwada, Shyuichi
dc.contributor.author Kitazawa, Haruki
dc.contributor.author Rose, M. T.
dc.contributor.author McConochie, H. R.
dc.contributor.author Okano, Hideyuki
dc.contributor.author Yamaguchi, Takahiro
dc.contributor.author Aso, Hisashi
dc.date.accessioned 2009-05-21T11:31:06Z
dc.date.available 2009-05-21T11:31:06Z
dc.date.issued 2008-08-01
dc.identifier.citation Kanaya , T , Miyazawa , K , Takakura , I , Itani , W , Watanabe , K , Ohwada , S , Kitazawa , H , Rose , M T , McConochie , H R , Okano , H , Yamaguchi , T & Aso , H 2008 , ' Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage ' American Journal of Physiology: Gastrointestinal and Liver Physiology , vol 295 , no. 2 , pp. G273-G284 . en
dc.identifier.issn 1522-1547
dc.identifier.other PURE: 102598
dc.identifier.other dspace: 2160/2314
dc.identifier.uri http://hdl.handle.net/2160/2314
dc.identifier.uri http://ajpgi.physiology.org/cgi/content/abstract/295/2/G273 en
dc.description Kanaya, T., Miyazawa, K., Takakura, I., Itani, W., Watanabe, K., Ohwada, S., Kitazawa, H., Rose, M.T., McConochie, H.R., Okano, H., Yamaguchi, T., Aso, H. (2008). Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage. American Journal of Physiology (Gastrointestinal and Liver Physiology), 295, G273-G284 KEYWORDS: M cell; MIE cell; follicle-associated epithelium; Peyer's patch; small intestine IMPF: 03.59 RONO: 00 en
dc.description.abstract M cells are a kind of intestinal epithelial cell in the follicle-associated epithelium of Peyer's patches. These cells can transport antigens and microorganisms into underlying lymphoid tissues. Despite the important role of M cells in mucosal immune responses, the origin and mechanisms of differentiation as well as cell death of M cells remain unclear. To clarify the mechanism of M cell differentiation, we established a novel murine intestinal epithelial cell line (MIE) from the C57BL/6 mouse. MIE cells grow rapidly and have a cobblestone morphology, which is a typical feature of intestinal epithelial cells. Additionally, they express cytokeratin, villin, cell-cell junctional proteins, and alkaline phosphatase activity and can form microvilli. Their expression of Musashi-1 antigen indicates that they may be close to intestinal stem cells or transit-amplifying cells. MIE cells are able to differentiate into the M cell lineage following coculture with intestinal lymphocytes, but not with Peyer's patch lymphocytes (PPL). However, PPL costimulated with anti-CD3/CD28 MAbs caused MIE cells to display typical features of M cells, such as transcytosis activity, the disorganization of microvilli, and the expression of M cell markers. This transcytosis activity of MIE cells was not induced by T cells isolated from PPL costimulated with the same MAbs and was reduced by the depletion of the T cell population from PPL. A mixture of T cells treated with MAbs and B cells both from PPL led MIE cells to differentiate into M cells. We report here that MIE cells have the potential ability to differentiate into M cells and that this differentiation required activated T cells and B cells. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof American Journal of Physiology: Gastrointestinal and Liver Physiology en
dc.subject M cell en
dc.subject MIE cell en
dc.subject follicle-associated epithelium en
dc.subject Peyer's patch en
dc.subject small intestine en
dc.title Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1152/ajpgi.00378.2007
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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