Show simple item record Kanaya, Takashi Miyazawa, Kohtaro Takakura, Ikuro Itani, Wataru Watanabe, Kouichi Ohwada, Shyuichi Kitazawa, Haruki Rose, M. T. McConochie, H. R. Okano, Hideyuki Yamaguchi, Takahiro Aso, Hisashi 2009-05-21T11:31:06Z 2009-05-21T11:31:06Z 2008-08-01
dc.identifier.citation Kanaya , T , Miyazawa , K , Takakura , I , Itani , W , Watanabe , K , Ohwada , S , Kitazawa , H , Rose , M T , McConochie , H R , Okano , H , Yamaguchi , T & Aso , H 2008 , ' Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage ' American Journal of Physiology - Gastrointestinal and Liver Physiology , vol 295 , no. 2 , pp. G273-G284 . DOI: 10.1152/ajpgi.00378.2007 en
dc.identifier.issn 1522-1547
dc.identifier.other PURE: 102598
dc.identifier.other PURE UUID: 72b56e14-a148-434d-b34b-a7fe3ceca429
dc.identifier.other dspace: 2160/2314
dc.identifier.other DSpace_20121128.csv: row: 1688
dc.identifier.other IBERS: 0000017523
dc.identifier.other Ibers_20121112_1204.csv: row: 201
dc.identifier.other RAD: 2896
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 1410
dc.identifier.other Scopus: 52449134022
dc.identifier.uri en
dc.description Kanaya, T., Miyazawa, K., Takakura, I., Itani, W., Watanabe, K., Ohwada, S., Kitazawa, H., Rose, M.T., McConochie, H.R., Okano, H., Yamaguchi, T., Aso, H. (2008). Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage. American Journal of Physiology (Gastrointestinal and Liver Physiology), 295, G273-G284 KEYWORDS: M cell; MIE cell; follicle-associated epithelium; Peyer's patch; small intestine IMPF: 03.59 RONO: 00 en
dc.description.abstract M cells are a kind of intestinal epithelial cell in the follicle-associated epithelium of Peyer's patches. These cells can transport antigens and microorganisms into underlying lymphoid tissues. Despite the important role of M cells in mucosal immune responses, the origin and mechanisms of differentiation as well as cell death of M cells remain unclear. To clarify the mechanism of M cell differentiation, we established a novel murine intestinal epithelial cell line (MIE) from the C57BL/6 mouse. MIE cells grow rapidly and have a cobblestone morphology, which is a typical feature of intestinal epithelial cells. Additionally, they express cytokeratin, villin, cell-cell junctional proteins, and alkaline phosphatase activity and can form microvilli. Their expression of Musashi-1 antigen indicates that they may be close to intestinal stem cells or transit-amplifying cells. MIE cells are able to differentiate into the M cell lineage following coculture with intestinal lymphocytes, but not with Peyer's patch lymphocytes (PPL). However, PPL costimulated with anti-CD3/CD28 MAbs caused MIE cells to display typical features of M cells, such as transcytosis activity, the disorganization of microvilli, and the expression of M cell markers. This transcytosis activity of MIE cells was not induced by T cells isolated from PPL costimulated with the same MAbs and was reduced by the depletion of the T cell population from PPL. A mixture of T cells treated with MAbs and B cells both from PPL led MIE cells to differentiate into M cells. We report here that MIE cells have the potential ability to differentiate into M cells and that this differentiation required activated T cells and B cells. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof American Journal of Physiology - Gastrointestinal and Liver Physiology en
dc.rights en
dc.subject M cell en
dc.subject MIE cell en
dc.subject follicle-associated epithelium en
dc.subject Peyer's patch en
dc.subject small intestine en
dc.title Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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