Effect of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids in steers fed grass or red clover silages

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dc.contributor.author Lee, M. R. F.
dc.contributor.author Shingfield, Kevin John
dc.contributor.author Tweed, J. K. S.
dc.contributor.author Toivonen, V.
dc.contributor.author Huws, S. A.
dc.contributor.author Scollan, N. D.
dc.date.accessioned 2009-05-21T12:14:20Z
dc.date.available 2009-05-21T12:14:20Z
dc.date.issued 2008-12
dc.identifier.citation Lee , M R F , Shingfield , K J , Tweed , J K S , Toivonen , V , Huws , S A & Scollan , N D 2008 , ' Effect of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids in steers fed grass or red clover silages ' Animal , vol 2 , no. 12 , pp. 1859-1869 . , 10.1017/S1751731108002899 en
dc.identifier.issn 1751-7311
dc.identifier.other PURE: 102768
dc.identifier.other dspace: 2160/2323
dc.identifier.uri http://hdl.handle.net/2160/2323
dc.description Lee, M. R. F., Shingfield, K. J., Tweed, J. K. S., Toivonen, V., Huws, S. A., Scollan, N. D. (2008). Effect of fish oil on ruminal lipid metabolism in steers fed grass or red clover silages. Animal, 2, (12), pp. 1859-1869 IMPF: 00.99 RONO: 1920 5112 en
dc.description.abstract Red clover and fish oil (FO) are known to alter ruminal lipid biohydrogenation leading to an increase in the polyunsaturated fatty acid (PUFA) and conjugated linoleic acid (CLA) content of ruminant-derived foods, respectively. The potential to exploit these beneficial effects were examined using eight Hereford × Friesian steers fitted with rumen and duodenal cannulae. Treatments consisted of grass silage or red clover silage fed at 90% of ad libitum intake and FO supplementation at 0, 10, 20 or 30 g/kg diet dry matter (DM). The experiment was conducted with two animals per FO level and treatments formed extra-period Latin squares. Flows of fatty acids at the duodenum were assessed using ytterbium acetate and chromium ethylene diamine tetra-acetic acid as indigestible markers. Intakes of DM were higher (P <0.001) for red clover silage than grass silage (5.98 v. 5.09 kg/day). There was a linear interaction effect (P = 0.004) to FO with a reduction in DM intake in steers fed red clover silage supplemented with 30 g FO/kg diet DM. Apparent ruminal biohydrogenation of C18:2n-6 and C18:3n-3 were lower (P <0.001) for red clover silage than grass silage (0.83 and 0.79 v. 0.87 and 0.87, respectively), whilst FO increased the extent of biohydrogenation on both diets. Ruminal biohydrogenation of C20:5n-3 and C22:6n-3 was extensive on both silage diets, averaging 0.94 and 0.97, respectively. Inclusion of FO in the diet enhanced the flow of total CLA leaving the rumen with an average across silages of 0.22, 0.31, 0.41 and 0.44 g/day for 0, 10, 20 or 30 g FO/kg, respectively, with a linear interaction effect between the two silages (P = 0.03). FO also showed a dose-dependent increase in the flow of trans-C18:1 intermediates at the duodenum from 4.6 to 15.0 g/day on grass silage and from 9.4 to 22.5 g/day for red clover silage. Concentrations of trans-C18:1 with double bonds from Δ4-16 in duodenal digesta were all elevated in response to FO in both diets, with trans-11 being the predominant isomer. FO inhibited the complete biohydrogenation of dietary PUFA on both diets, whilst red clover increased the flow of C18:2n-6 and C18:3n-3 compared with grass silage. In conclusion, supplementing red clover silage-based diets with FO represents a novel nutritional strategy for enhancing the concentrations of beneficial fatty acids in ruminant milk and meat. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Animal en
dc.subject fish oil en
dc.subject red clover en
dc.subject ruminal biohydrogenation en
dc.subject trans fatty acids en
dc.subject conjugated linoleic acid en
dc.title Effect of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids in steers fed grass or red clover silages en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1017/S1751731108002899
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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