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dc.contributor.author Lee, Michael R. F.
dc.contributor.author Sulivan, Michael L.
dc.contributor.author Tweed, John K. S.
dc.contributor.author Scollan, Nigel D.
dc.date.accessioned 2009-05-21T12:18:52Z
dc.date.available 2009-05-21T12:18:52Z
dc.date.issued 2009-05-21
dc.identifier.citation Lee , M R F , Sulivan , M L , Tweed , J K S & Scollan , N D 2009 , ' Ruminal micro-organisms do not adapt to increase utilization of polyphenol oxidase protected red clover protein and glycerol-based lipid ' Unknown Journal , pp. 2479-2485 . en
dc.identifier.other PURE: 111649
dc.identifier.other dspace: 2160/2325
dc.identifier.uri http://hdl.handle.net/2160/2325
dc.description Lee, M. R. F., Tweed, J. K. S., Scollan, N. D., Sullivan, M. L. (2008). Ruminal micro-organisms do not adapt to increase utilization of polyphenol oxidase protected red clover protein and glycerol-based lipid.  Journal of the Science of Food and Agriculture, 88, (14), 2479-2485. en
dc.description.abstract BACKGROUND: The enzyme polyphenol oxidase (PPO) reduces the extent of proteolysis and lipolysis within red clover fed to ruminants with subsequent increases in the efficiency of N utilization and the level of beneficial polyunsaturated fatty acids in their products (meat and milk). It has also been reported that red clover feeding alters the rumen microbial population compared to grass feeding. This study investigated whether the observed shifts in the microbial population of the rumen when ruminants are fed red clover silage (RC) as opposed to grass silage (G) represented an adaptation by the micro-organisms to increase the utilization of PPO-protected protein and glycerol-based lipid. RESULTS: The experiment consisted of two periods where ruminally fistulated dairy cows were offered either RC or G for 2 weeks, followed by collection of rumen fluid, which was then used in in vitro incubations to investigate lipolysis and proteolysis over time in plant material derived from red clover plants with either wild type PPO expression (PPO+) or PPO expression reduced to undetectable levels by gene silencing (PPO-). Proteolysis and lipolysis (P <0.05) were lower after 24 h of incubation in the PPO+ treatment than the PPO- treatment irrespective of rumen fluid. Biohydrogenation of C18 polyunsaturated fatty acids was also lower on the PPO+ treatment than the PPO- treatment, with no effect of rumen fluid. CONCLUSION: These results suggest that microbial changes to red clover feeding did not result in an increased ability of the micro-organisms in the present study to utilize either PPO-protected protein or glycerol-based lipid. en
dc.format.extent 7 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title Ruminal micro-organisms do not adapt to increase utilization of polyphenol oxidase protected red clover protein and glycerol-based lipid en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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