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dc.contributor.author Hirst, W. M.
dc.contributor.author Parveen, Ifat
dc.contributor.author Moorby, Jon M.
dc.contributor.author Morris, S. Michael
dc.contributor.author Fraser, Mariecia D.
dc.date.accessioned 2009-05-21T12:20:45Z
dc.date.available 2009-05-21T12:20:45Z
dc.date.issued 2009-05-21
dc.identifier.citation Hirst , W M , Parveen , I , Moorby , J M , Morris , S M & Fraser , M D 2009 , ' Profiling of plasma and faeces by FT-IR to differentiate between heathland plant diets offered to zero-grazed sheep ' Animal Feed Science and Technology , pp. 1-2 . en
dc.identifier.other PURE: 111742
dc.identifier.other dspace: 2160/2326
dc.identifier.uri http://hdl.handle.net/2160/2326
dc.description Parveen, I., Moorby, J. M., Hirst, W. M., Morris, S. M., Fraser, M. D. (2008). Profiling of plasma and faeces by FT-IR to differentiate between heathland plant diets offered to zero-grazed sheep. Animal Feed Science and Technology, 144, (1-2), 65-81. Keywords: Fourier-transform infrared spectroscopy; Principal component analysis; Multinomial regression; Heathland plants; Herbivory en
dc.description.abstract To investigate the relationships between plant composition in a complex diet mixture and intake by sheep, blood and faeces were analysed by Fourier transform mid-infrared (FT-IR) spectroscopy. Each of four mature Welsh Mountain ewes was maintained, for a period of 9 days, on one of three diets comprising a mixture of heathland plants containing either 100 g/kg (H100), 200 g/kg (H200) or 300 g/kg (H300) heather (Calluna vulgaris) in the fresh matter. Spectra of blood and faeces from each animal were acquired and the data analysed using principal component analysis (PCA) and hierarchical cluster analysis (HCA). We also investigated multinomial regression as an extension beyond the pattern recognition state. Animals offered the H300 diet could be consistently separated from animals fed on the H100 and H200 diets by PCA and HCA of plasma data, and these results were consistent with animals on the H300 diet consuming greater quantities of heather. Using faecal spectra, PCA and HCA discriminated between animals on the H200 diet and those on the H100 and H300 diets. Analysis of the plasma and faecal data by the multinomial model indicated that when PCs 4, 5 and 6 of the plasma data and PCs 3 and 4 of the faecal data were selected as covariates, leave-one-out cross-validation (LOO-CV) achieved an accurate dietary classification for 9 out of the 11 animals. In the present study, we have shown the ability to discriminate animals offered closely related diets on the basis of metabolic fingerprints of plasma and faeces. en
dc.format.extent 2 en
dc.language.iso eng
dc.relation.ispartof Animal Feed Science and Technology en
dc.title Profiling of plasma and faeces by FT-IR to differentiate between heathland plant diets offered to zero-grazed sheep en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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