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dc.contributor.author Harding, Victoria
dc.contributor.author Kaderbhai, Naheed N.
dc.contributor.author Kaderbhai, Mustak A.
dc.date.accessioned 2009-05-21T12:36:40Z
dc.date.available 2009-05-21T12:36:40Z
dc.date.issued 2008-08-01
dc.identifier.citation Harding , V , Kaderbhai , N N & Kaderbhai , M A 2008 , ' Signal peptidase I-mediated processing of an engineered mammalian cytochrome b5 precursor is an exocytoplasmic post-translocational event in Escherichia coli ' Molecular Membrane Biology , vol 25 , no. 5 , pp. 388-399 . , 10.1080/09687680802154799 en
dc.identifier.issn 1464-5203
dc.identifier.other PURE: 102964
dc.identifier.other dspace: 2160/2330
dc.identifier.uri http://hdl.handle.net/2160/2330
dc.description Kaderbhai, N. N., Harding, V., Kaderbhai, M. A. (2008). Signal peptidase I-mediated processing of an engineered mammalian cytochrome b5 precursor is an exocytoplasmic post-translocational event in Escherichia coli.  Molecular Membrane Biology, 25, (5), 388-399. IMPF: 03.60 RONO: 00 en
dc.description.abstract Proteins destined for translocation across the prokaryotic cytoplasmic membrane are synthesized as precursors carrying transient N-terminal extensions known as signal sequences. They facilitate initial engagement of precursor proteins with the sec-dependent translocase to initiate active threading of the polypeptide across the membrane. The translocated precursor is then processed by a transcytoplasmic signal peptidase anchored to the inner membrane. The temporal nature of cleavage of the signal sequence during pre-protein translocation has remained elusive. Using an engineered mammalian cytochrome b5 precursor we demonstrate that the signal peptide processing in Escherichia coli is an event that can occur after almost complete exocytoplasmic translocation of the preprotein is accomplished. We discuss implications of the findings in light of the known working model of sec-dependent pre-protein translocon. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof Molecular Membrane Biology en
dc.subject Protein translocation en
dc.subject protein targeting en
dc.subject signal sequence processing signal peptidase I en
dc.subject cytochrome b5 en
dc.subject recombinant protein en
dc.title Signal peptidase I-mediated processing of an engineered mammalian cytochrome b5 precursor is an exocytoplasmic post-translocational event in Escherichia coli en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1080/09687680802154799
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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