Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidase

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dc.contributor.author Parveen, I.
dc.contributor.author Winters, A.
dc.contributor.author Threadgill, Michael D.
dc.contributor.author Hauck, B. D.
dc.contributor.author Morris, Phillip
dc.date.accessioned 2009-05-21T12:39:17Z
dc.date.available 2009-05-21T12:39:17Z
dc.date.issued 2008-11
dc.identifier.citation Parveen , I , Winters , A , Threadgill , M D , Hauck , B D & Morris , P 2008 , ' Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidase ' Phytochemistry , vol 69 , no. 16 , pp. 2799-2806 . en
dc.identifier.other PURE: 102987
dc.identifier.other dspace: 2160/2331
dc.identifier.uri http://hdl.handle.net/2160/2331
dc.description Parveen, I., Winters, A., Threadgill, M. D., Hauck, B. D., Morris, P. (2008). Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidase.  Phytochemistry, 69, (16), 2799-2806. IMPF: 02.95 RONO: 1910 3007 en
dc.description.abstract Polyphenol oxidase (PPO) activity has been reported in orchard grass (Dactylis glomerata); however, to date, no endogenous substrates have been identified. In the present study, we report the isolation and structural elucidation of PPO substrates in this species. The free phenol fraction was extracted, separated by reverse-phase chromatography and six potential substrates, including two hydroxycinnamate esters, were identified by UV spectrometry, electrospray ionisation-tandem mass spectrometry (LC?ESI-MSn) and 1D and 2D NMR analyses (1H NMR, 13C NMR, DEPT, COSY, HMQC and HMBC). Furthermore, three caffeoylquinic acids (3-CQA, 4-CQA and 5-CQA) were identified by comparison of their spectral data (ESI-MS) with those of known compounds and literature data. Five of these compounds were demonstrated to be substrates for orchard grass PPO. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Phytochemistry en
dc.title Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidase en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.phytochem.2008.08.019
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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