A High-throughput Method for the Quantification of Proanthocyanidins in Forage Crops and its Application in Assessing Variation in Condensed Tannin Content in Breeding Programmes for Lotus corniculatus and Lotus uliginosus

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dc.contributor.author Marshall, Athole
dc.contributor.author Bryant, David
dc.contributor.author Latypova, Galina
dc.contributor.author Hauck, Barbara
dc.contributor.author Olyott, Phil
dc.contributor.author Morris, Phillip
dc.contributor.author Robbins, Mark
dc.date.accessioned 2009-05-21T13:50:33Z
dc.date.available 2009-05-21T13:50:33Z
dc.date.issued 2008
dc.identifier.citation Marshall , A , Bryant , D , Latypova , G , Hauck , B , Olyott , P , Morris , P & Robbins , M 2008 , ' A High-throughput Method for the Quantification of Proanthocyanidins in Forage Crops and its Application in Assessing Variation in Condensed Tannin Content in Breeding Programmes for Lotus corniculatus and Lotus uliginosus ' Journal of Agricultural and Food Chemistry , vol 56 , no. 3 , pp. 974-981 . en
dc.identifier.issn 0021-8561
dc.identifier.other PURE: 103540
dc.identifier.other dspace: 2160/2351
dc.identifier.uri http://hdl.handle.net/2160/2351
dc.description IMPF: 02.56 RONO: 1330 4206; 1910 6742; 1910 3007 en
dc.description.abstract Lotus corniculatus and Lotus uliginosus are agronomically important forage crops used in ruminant livestock production. The condensed tannin (CT) content, dry matter (DM) production, and persistence of these species are key characteristics of interest for future exploitation of these crops. Here we present field data on 19 varieties of L. corniculatus, 2 varieties of L. uliginosus and, additionally, a glasshouse experiment using 6 varieties of L. corniculatus and 2 varieties of L. uliginosus. Current methods for the quantification of condensed tannins in crop species are slow and labor intensive and are generally based upon polymer hydrolysis following the extraction of chlorophyll in a liquid phase. Presented here is a high-throughput protocol for condensed tannin quantification suitable for microtiter plates based upon the precipitation of condensed tannin polymers in complex with bovine serum albumin (BSA) with subsequent hydrolysis of precipates using butan 1-ol/hydrochloric acid. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Journal of Agricultural and Food Chemistry en
dc.subject Lotus en
dc.subject condensed tannins en
dc.subject proanthocyanidins en
dc.subject protein precipitation en
dc.subject forage quality en
dc.title A High-throughput Method for the Quantification of Proanthocyanidins in Forage Crops and its Application in Assessing Variation in Condensed Tannin Content in Breeding Programmes for Lotus corniculatus and Lotus uliginosus en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1021/jf072330+
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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