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dc.contributor.author Croxford, Adam E.
dc.contributor.author Lopez, C. M. R.
dc.contributor.author Wilkinson, Michael J.
dc.date.accessioned 2009-05-22T08:42:46Z
dc.date.available 2009-05-22T08:42:46Z
dc.date.issued 2008-07-01
dc.identifier.citation Croxford , A E , Lopez , C M R & Wilkinson , M J 2008 , ' High-resolution melt analysis for SNP discovery, linkage mapping and analysis of DNA methylation ' Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology , vol 150 , no. 3 , pp. S49-S50 . , 10.1016/j.cbpa.2008.04.035 en
dc.identifier.issn 1095-6433
dc.identifier.other PURE: 104137
dc.identifier.other dspace: 2160/2378
dc.identifier.uri http://hdl.handle.net/2160/2378
dc.description Lopez, C. M. R., Croxford, A. E., Wilkinson, M. J. (2008). High-resolution melt analysis for SNP discovery, linkage mapping and analysis of DNA methylation  Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology, Abstracts, Annual Meeting of the Society-for-Experimental-Biology, Marseille, FRANCE, JUL 06-10, 2008, 150, (3), S49-S50 en
dc.description.abstract High Resolution Melt analysis (HRM) is a closed-tube method of genotyping that does not require use of fluorescent probes, fragment fractionation or amplicon sequence information. Recent advancements in florescent-detection instruments (such as the Corbett Rotor-Gene 6000) and the use of fully saturating intercalating dyes have made HRM analysis considerably more sensitive. The flexibility of the system allows it to be adapted for a wide range of uses including SNP genotyping, mutation detection, screening for loss of heterozygosity, DNA fingerprinting, characterization of haplotype blocks, species classification, somatically acquired mutations studies, linkage and physical mapping, and DNA methylation analysis. Here, we describe the first use of high-resolution melt analysis to generate STS markers based on Single Nucleotide Polymorphisms (SNPs) and microsatellite length polymorphisms for use in linkage mapping, using white lupin (Lupinus albus, x = 25) as a case study. The described strategy is rapid and low-cost, and circumvents the need for labeled primers or amplicon fractionation. We also illustrate the use of HRM analysis for the detection and/or quantification of the presence of, and relative abundance of, methylated nucleic acid bases within the double-stranded molecule without any prior chemical modification of the target DNA. en
dc.format.extent 5 en
dc.language.iso eng
dc.relation.ispartof Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology en
dc.title High-resolution melt analysis for SNP discovery, linkage mapping and analysis of DNA methylation en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.cbpa.2008.04.035
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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