Chromosome pairing in allotetraploid hybrids of Festuca pratensis × Lolium perenne revealed by genomic in situ hybridization (GISH)

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dc.contributor.author Jones, R. N.
dc.contributor.author Zwierzykowski, Zbigniew
dc.contributor.author Zwierzykowska, Elżbieta
dc.contributor.author Taciak, Magdalena
dc.contributor.author Kosmala, Arkadiusz
dc.contributor.author Krajewski, Paweł
dc.date.accessioned 2009-05-26T09:18:30Z
dc.date.available 2009-05-26T09:18:30Z
dc.date.issued 2008-06-01
dc.identifier.citation Jones , R N , Zwierzykowski , Z , Zwierzykowska , E , Taciak , M , Kosmala , A & Krajewski , P 2008 , ' Chromosome pairing in allotetraploid hybrids of Festuca pratensis × Lolium perenne revealed by genomic in situ hybridization (GISH) ' Chromosome Research , vol 16 , no. 4 , pp. 575-585 . en
dc.identifier.other PURE: 104450
dc.identifier.other dspace: 2160/2388
dc.identifier.uri http://hdl.handle.net/2160/2388
dc.description Zwierzykowski, Z., Zwierzykowska, E., Taciak, M., Jones, R. N., Kosmala, A., Krajewski, P. (2008). Chromosome pairing analysis in allotetraploid hybrids of Festuca pratensis x Lolium perenne using genomic in situ (GISH).  Chromosome Research, 16, (4), 575-585. IMPF: 03.40 RONO: 00 en
dc.description.abstract Genomic in situ hybridization (GISH) was used to make a detailed study of chromosome pairing at metaphase I (MI) of meiosis in six F1 hybrid plants of the allotetraploid Festuca pratensis × Lolium perenne (2n = 4x = 28; genomic constitution FpFpLpLp). The mean chromosome configurations for all hybrids analysed were 1.13 univalents + 11.51 bivalents + 0.32 trivalents + 0.72 quadrivalents, and the mean chiasma frequency was 21.96 per cell. GISH showed that pairing was predominantly intragenomic, with mean numbers of L. perenne (Lp/Lp) and F. pratensis (Fp/Fp) bivalents being virtually equal at 5.41 and 5.48 per cell, respectively. Intergenomic pairing between Lolium and Festuca chromosomes was observed in 33.3% of Lp/Fp bivalents (0.62 per cell), in 79.7% of trivalents – Lp/Lp/Fp and Lp/Fp/Fp (0.25 per cell), and in 98.4% of quadrivalents – Lp/Lp/Fp/Fp and Lp/Lp/Lp/Fp (0.71 per cell). About 4.0% of the total chromosome complement analysed remained as univalents, an average 0.68 Lp and 0.45 Fp univalents per cell. It is evident that in these hybrids there is opportunity for recombination to take place between the two component genomes, albeit at a low level, and this is discussed in the context of compromising the stability of Festulolium hybrid cultivars and accounting for the drift in the balance of the genomes over generations. We speculate that genotypic differences between hybrids could permit selection for pairing control, and that preferences for homologous versus homoeologous centromeres in their spindle attachments and movement to the poles at anaphase I could form the basis of a mechanism underlying genome drift. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Chromosome Research en
dc.subject allopolyploids en
dc.subject chromosome pairing en
dc.subject Festuca pratensis × Lolium perenne hybrids en
dc.subject genomic in situ hybridization (GISH) en
dc.title Chromosome pairing in allotetraploid hybrids of Festuca pratensis × Lolium perenne revealed by genomic in situ hybridization (GISH) en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s10577-008-1198-6
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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