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dc.contributor.author Sasanuma, Tsuneo
dc.contributor.author Sehgal, Deepmala
dc.contributor.author Raina, Soom Nath
dc.contributor.author Sasakuma, Tetsuo
dc.contributor.author Rajpal, Vijay Rani
dc.date.accessioned 2009-06-04T08:46:37Z
dc.date.available 2009-06-04T08:46:37Z
dc.date.issued 2008-07-23
dc.identifier.citation Sasanuma , T , Sehgal , D , Raina , S N , Sasakuma , T & Rajpal , V R 2008 , ' Assaying polymorphism at DNA sequence level for new and novel genetic diversity diagnostics of the safflower (Carthamus tinctorius L.) ' Genetica , vol 135 , no. 3 , pp. 457-470 . , 10.1007/s10709-008-9292-4 , 10.1007/s10709-008-9292-4 en
dc.identifier.issn 1573-6857
dc.identifier.other PURE: 105898
dc.identifier.other dspace: 2160/2429
dc.identifier.uri http://hdl.handle.net/2160/2429
dc.description Sehgal, D., Rajpal, V. R., Raina, S. N., Sasanuma, T., Sasakuma, T. (2008). Assaying polymorphism at DNA sequence level for new and novel genetic diversity diagnostics of the safflower (Carthamus tinctorius L.).  Genetica, 135, 457-470. IMPF: 02.09 Sponsorship: Department of Biotechnology and Council of Scientific and Industrial Research, Ministry of Science and Technology, Government of India, and special coordination funds of Science and Technology Agency of the Japan Government. en
dc.description.abstract Carthamus tinctorius (2n = 2x = 24), commonly known as safflower, is widely cultivated in agricultural production systems of Asia, Europe, Australia, and the Americas as a source of high quality vegetable and industrial oil. Twenty-two RAPD primers, 18 SSR primers, and 10 AFLP primer combinations were used to assess: (1) the genetic diversity of 85 accessions (originating from 24 countries) representing global germplasm variability of safflower and (2) the interrelationships among safflower ‘centers of similarity’ or ‘regional gene pools’ proposed earlier. The RAPD and SSR primers and AFLP primer combinations revealed 57.6, 68.0, and 71.2% polymorphism, respectively, among 111, 72, and 330 genetic loci amplified from the accessions. The sum of effective number of alleles (66.44), resolving power (59.16), and marker index (51.3) explicitly revealed the relative superiority of AFLP as a marker system in uncovering variation in safflower. Overall, AFLP markers could recognize ‘centers of similarity’ or ‘regional gene pools’. Analysis of molecular variance and Shannon’s information index provided corroborating evidences for the present and previous studies that concluded fragmentation of safflower gene pool into many gene pools. Divergent directional selection is likely to have played an important role in shaping the diversity. From the practical applications standpoint, the diversity of Iran–Afghanistan gene pool is very high, equivalent to the total diversity of the species. The Far East gene pool is the least diverse. The present comprehensive input, first of its own kind in safflower, will assist marker based improvement programmes in the crop. en
dc.format.extent 14 en
dc.language.iso eng
dc.relation.ispartof Genetica en
dc.title Assaying polymorphism at DNA sequence level for new and novel genetic diversity diagnostics of the safflower (Carthamus tinctorius L.) en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s10709-008-9292-4
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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