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dc.contributor.author Minashima, T.
dc.contributor.author Hagino, A.
dc.contributor.author Ohwada, Shyuichi
dc.contributor.author Suzuki, K.
dc.contributor.author Rose, Michael T.
dc.contributor.author Watanabe, K.
dc.contributor.author Yamaguchi, T.
dc.contributor.author Aso, Hisashi
dc.contributor.author Sanosaka, M.
dc.date.accessioned 2009-06-04T10:06:10Z
dc.date.available 2009-06-04T10:06:10Z
dc.date.issued 2009-06-04
dc.identifier.citation Minashima , T , Hagino , A , Ohwada , S , Suzuki , K , Rose , M T , Watanabe , K , Yamaguchi , T , Aso , H & Sanosaka , M 2009 , ' A combination of octanoate and oleate promotes in vitro differentiation of porcine intramuscular adipocytes ' Unknown Journal , pp. 285-292 . en
dc.identifier.other PURE: 114501
dc.identifier.other dspace: 2160/2443
dc.identifier.uri http://hdl.handle.net/2160/2443
dc.description Sanosaka, M., Minashima, T., Suzuki, K., Watanabe, K., Ohwada, S., Hagino, A., Rose, M. T., Yamaguchi, T., Aso, H. (2008). A combination of octanoate and oleate promotes in vitro differentiation of porcine intramuscular adipocytes.  Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 149, (2), 285-292. en
dc.description.abstract To understand the relationship between intramuscular adipogenesis in the pig and the supply fatty acids, we established a clonal porcine intramuscular preadipocyte (PIP) line from the marbling muscle tissue of female Duroc pig. Confluent PIP cells exhibited a fibroblastic appearance. Their adipogenic ability was investigated using confluent PIP cells after exchanging growth medium for adipogenic medium containing 50 ng/mL insulin, 0.25 ¿M dexamethasone, 2 mM octanoate, and 200 ¿M oleate. Appropriate concentrations of octanoate and oleate for the induction of adipogenesis were determined from the ability of cells to accumulate lipid and the toxicity of fatty acids. When cells were cultured in differentiation medium for 8 days, large numbers of lipid droplets were observed in differentiated PIP cells, and their cytosolic TG content increased in a time-dependent manner. While oleate only induced the expression of PPAR¿ mRNA, but not that of C/EBP¿, octanoate significantly induced the expression of both PPAR¿ and C/EBP¿ mRNA. Octanoate and oleate accelerated the inducing effect of insulin and dexamethasone on the expression of aP2 mRNA. These results indicate that a combination of octanoate and oleate synergistically induced PIP adipogenesis, and that the stimulation of octanoate was essential to the trigger for the adipogenesis in PIP cells. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title A combination of octanoate and oleate promotes in vitro differentiation of porcine intramuscular adipocytes en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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