The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees

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dc.contributor.author Allainguillaume, Joel
dc.contributor.author Quainoo, A. K.
dc.contributor.author Wetten, A. C.
dc.date.accessioned 2009-06-04T11:07:17Z
dc.date.available 2009-06-04T11:07:17Z
dc.date.issued 2008-04-01
dc.identifier.citation Allainguillaume , J , Quainoo , A K & Wetten , A C 2008 , ' The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees ' Journal of Virological Methods , vol 149 , no. 1 , pp. 91-96 . , 10.1016/j.jviromet.2008.01.007 en
dc.identifier.other PURE: 106228
dc.identifier.other dspace: 2160/2457
dc.identifier.uri http://hdl.handle.net/2160/2457
dc.description Quainoo, A. K., Wetten, A. C., Allainguillaume, J. (2008). The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees.  Journal of Virological Methods, 149, (1), 91-96. IMPF: 02.08 RONO: 00 en
dc.description.abstract Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSSV in all the callus tissues induced from the CSSV-infected Amelonado cocoa trees (T1, T2 and T4). The virus was transmitted to primary somatic embryos induced from the infected callus tissues at the rate of 10 (19%), 18 (14%) and 16 (15%) for T1, T2 and T4, respectively. Virus free primary somatic embryos from the infected callus tissues converted into plantlets tested CSSV negative by PCR/capillary electrophoresis 2 years after weaning. Secondary somatic embryos induced from the CSSV-infected primary somatic embryos revealed the presence of viral fragments at the rate of 4 (4%) and 9 (9%) for T2 and T4, respectively. Real-time PCR revealed 23 of the 24 secondary somatic embryos contained no detectable virus. Based on these findings, it is proposed that progressive elimination of the CSSV in infected cocoa trees occurred from primary embryogenesis to secondary embryogenesis. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Journal of Virological Methods en
dc.subject Primary embryogenesis en
dc.subject PCR/capillary electrophoresis en
dc.subject Real-time polymerase chain reaction en
dc.subject Somatic embryos en
dc.subject Secondary embryogenesis en
dc.subject Cocoa swollen shoot virus (CSSV) en
dc.title The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.jviromet.2008.01.007
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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