Protein-protein interactions between two-component system transmitter and receiver domains of Myxococcus xanthus

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dc.contributor.author Whitworth, David E.
dc.contributor.author Hawkins, P. F.
dc.contributor.author Millard, A.
dc.contributor.author Hodgson, David A.
dc.date.accessioned 2009-06-04T14:59:26Z
dc.date.available 2009-06-04T14:59:26Z
dc.date.issued 2009-06-04
dc.identifier.citation Whitworth , D E , Hawkins , P F , Millard , A & Hodgson , D A 2009 , ' Protein-protein interactions between two-component system transmitter and receiver domains of Myxococcus xanthus ' Unknown Journal , pp. 1839-1842 . en
dc.identifier.other PURE: 115021
dc.identifier.other dspace: 2160/2470
dc.identifier.uri http://hdl.handle.net/2160/2470
dc.description Whitworth, D. E., Millard, A., Hodgson, D. A., Hawkins, P. F. (2008). Protein-protein interactions between two-component system transmitter and receiver domains of Myxococcus xanthus.  Proteomics, 8, pp. 1839-1842 Keywords: Cluster analysis ¿ Interaction profiling ¿ Myxobacteria ¿ Protein-protein interactions ¿ Two-component systems en
dc.description.abstract We present a novel dataset assessing the specificity of protein-protein interactions between 69 transmitter and receiver domains from two-component system (TCS)-signalling pathways. TCS require a conserved protein-protein interaction between partner transmitter and receiver domains for signal transduction. The complex prokaryote Myxococcus xanthus possesses an unusually large number of TCS genes, many of which have no obvious interaction partners. Interactions between TCS domains of M. xanthus were assessed using a yeast two-hybrid assay, in which domains were expressed as both bait and prey translational fusions. LacZ production was monitored as an indicator of protein-protein interaction, and the strength of interactions classified as weak, medium or strong. Two-hundred and fifty-five transmitter-receiver domain interactions were observed (46 strong), allowing identification of potential signalling partners for individual M. xanthus TCS proteins. In addition, the dataset provides interesting meta information. For instance, many strong interactions were identified between different transmitter domain pairs (34) and receiver domain pairs (23), suggesting a surprisingly large degree of heterodimerisation of these domains. Proteins in our dataset that exhibited similar profiles of interactions, often shared a similar biological function, suggesting that interaction profiles can provide information on biological function, even considering sets of homologous domains. en
dc.format.extent 4 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title Protein-protein interactions between two-component system transmitter and receiver domains of Myxococcus xanthus en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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