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dc.contributor.author T. en_US
dc.contributor.author Ann M. en_US
dc.contributor.author Mervyn O. en_US
dc.contributor.author Iain S. en_US
dc.contributor.author Torben en_US
dc.contributor.author Kerrie en_US
dc.contributor.author M. en_US
dc.contributor.author C. en_US
dc.date.accessioned 2009-07-09T08:41:54Z
dc.date.available 2009-07-09T08:41:54Z
dc.date.issued 2007-01 en_US
dc.identifier http://dx.doi.org/10.1007/s11032-006-9036-z en_US
dc.identifier.citation Lubberstedt , T , Thomas , A M , Humphreys , M O , Donnison , I S , Asp , T , Farrar , K , Xu , M & Christiansen , C 2007 , ' Construction of two Lolium perenne BAC libraries and identification of BACs containing candidate genes for disease resistance and forage quality ' Molecular Breeding , pp. 15-23 . , 10.1007/s11032-006-9036-z en_US
dc.identifier.other PURE: 108352 en_US
dc.identifier.other dspace: 2160/2588 en_US
dc.identifier.uri http://hdl.handle.net/2160/2588
dc.description.abstract Two BAC libraries were constructed for the forage and turf grass species Lolium perenne L. The libraries consisted of 98,304 and 101,376 BAC clones for L. perenne genotypes LTS18 and NV#20F1-30, respectively. The estimated average insert size of both libraries was approximately 100 Kb and L. perenne has a published haploid genome size of 2,034 Mb. Taken together, the two libraries represent almost 10 genome equivalents, so that there is a very high probability of any specific sequence being represented. BAC DNA was isolated and pooled to enable PCR-based screening of both libraries. In addition, BAC clones from the LTS18 genotype were replicated onto filters to enable hybridisation-based screening. To validate the libraries, primers were designed to 20 genes involved in the phenylpropanoid pathway, disease resistance candidate genes and laccases. These primers were used to screen both libraries to verify the genome coverage and to enable the identification of full-length gene and promoter sequences for subsequent single nucleotide polymorphism (SNP) analyses. These sequences will enable studies of gene function and regulation as well as the identification of efficient genetic markers for plant breeders to improve disease resistance and forage quality. en_US
dc.format.extent 9 en_US
dc.relation.ispartof Molecular Breeding en_US
dc.title Construction of two Lolium perenne BAC libraries and identification of BACs containing candidate genes for disease resistance and forage quality en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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