Ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis. Structure, expression analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in Lotus corniculatus

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dc.contributor.author Paolocci, Francesco
dc.contributor.author Robbins, Mark P.
dc.contributor.author Madeo, Laura
dc.contributor.author Arcioni, Sergio
dc.contributor.author Martens, Stefan
dc.contributor.author Damiani, Francesco
dc.date.accessioned 2009-07-09T11:16:40Z
dc.date.available 2009-07-09T11:16:40Z
dc.date.issued 2006-11-10
dc.identifier.citation Paolocci , F , Robbins , M P , Madeo , L , Arcioni , S , Martens , S & Damiani , F 2006 , ' Ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis. Structure, expression analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in Lotus corniculatus ' Plant Physiology , vol 143 , no. 1 , pp. 504-516 . en
dc.identifier.issn 0032-0889
dc.identifier.other PURE: 109050
dc.identifier.other dspace: 2160/2606
dc.identifier.uri http://hdl.handle.net/2160/2606
dc.identifier.uri http://www.plantphysiol.org/cgi/content/abstract/143/1/504 en
dc.description Paolocci, F., Robbins, M. P., Madeo, L., Arcioni, S., Martens, S., Damiani, F. (2007). Ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis. Structure, expression analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in Lotus corniculatus. Plant Physiology, 143, (1), 504-516 en
dc.description.abstract Proanthocyanidins (PAs) are plant secondary metabolites and are composed primarily of catechin and epicatechin units in higher plant species. Due to the ability of PAs to bind reversibly with plant proteins to improve digestion and reduce bloat, engineering this pathway in leaves is a major goal for forage breeders. Here, we report the cloning and expression analysis of anthocyanidin reductase (ANR) and leucoanthocyanidin 4-reductase (LAR), two genes encoding enzymes committed to epicatechin and catechin biosynthesis, respectively, in Lotus corniculatus. We show the presence of two LAR gene families (LAR1 and LAR2) and that the steady-state levels of ANR and LAR1 genes correlate with the levels of PAs in leaves of wild-type and transgenic plants. Interestingly, ANR and LAR1, but not LAR2, genes produced active proteins following heterologous expression in Escherichia coli and are affected by the same basic helix-loop-helix transcription factor that promotes PA accumulation in cells of palisade and spongy mesophyll. This study provides direct evidence that the same subclass of transcription factors can mediate the expression of the structural genes of both branches of PA biosynthesis. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Plant Physiology en
dc.title Ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis. Structure, expression analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in Lotus corniculatus en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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