The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis

H...............H

Show simple item record

dc.contributor.author Machowski, E.
dc.contributor.author Gordhan, B. G.
dc.contributor.author Sung, N.
dc.contributor.author Downing, Katrina J.
dc.contributor.author Tsenova, L.
dc.contributor.author Matsoso, L.
dc.contributor.author Kaplan, G.
dc.contributor.author Young, Michael
dc.contributor.author Kana, B. D.
dc.contributor.author Mizrahi, V.
dc.date.accessioned 2009-07-09T14:01:37Z
dc.date.available 2009-07-09T14:01:37Z
dc.date.issued 2007
dc.identifier.citation Machowski , E , Gordhan , B G , Sung , N , Downing , K J , Tsenova , L , Matsoso , L , Kaplan , G , Young , M , Kana , B D & Mizrahi , V 2007 , ' The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis ' . en
dc.identifier.other PURE: 108540
dc.identifier.other dspace: 2160/2615
dc.identifier.uri http://hdl.handle.net/2160/2615
dc.description Kana, B. D., Gordhan, B., Sung, N., Downing, K., Machowski, E., Matsoso, L., Tsenova, L., Young, M., Kaplan, G., Mizrahi, V. (2007). The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis. FASEB Journal, 21 (5), 446.2, A207. Sponsorship: Wellcome Trust (#065578)/ HHMI / NIH (RO1 AI 54338 and AI54361)/ Medical Research Council of South Africa /Columbia University-Southern African Fogarty AIDS International Research and Training Program (# 5 D43 TW00231, FIC, NIH) en
dc.description.abstract The ability of Mycobacterium tuberculosis to enter into a dormant state has resulted in one third of the world’s population being infected with latent tuberculosis. As such, the study of the molecular mechanisms that contribute to latency and reactivation is of utmost importance. M. tuberculosis encodes five resuscitation promoting factors, RpfA-E, which share homology with Micrococcus luteus Rpf, a muralytic enzyme implicated in reactivation of this organism from a dormant state. We have undertaken the combinatorial deletion of up to four of the five rpf genes from M. tuberculosis H37Rv to produce a set of deletion mutants that include three quadruple mutant strains that retain only rpfB, rpfD or rpfE. These strains were assessed for growth and survival in vitro and in macrophages and for virulence in mice. Our data indicated that although the quadruple mutants displayed normal growth and survival in liquid media and in human PBMCs, they showed reduced growth kinetics on solid media. Moreover, all three strains showed markedly reduced virulence in mice, with those carrying only rpfB or rpfD being unable to maintain chronic infection in vivo. These findings, together with preliminary data on the expression of the remaining rpf genes in the set of deletion mutant strains, suggest that the Rpf proteins of M. tuberculosis may have discrete functions. en
dc.language.iso eng
dc.title The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis en
dc.type Text en
dc.type.publicationtype Conference contribution en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Non peer reviewed en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Cadair


Advanced Search

Browse

My Account